SiR-Hoechst is a far-red DNA stain for live-cell nanoscopy.
SiR-Hoechst is a far-red DNA stain for live-cell nanoscopy.
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DOI：
10.1038/ncomms9497
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发表时间：
2015-10-01
影响因子：
16.6
通讯作者：
Johnsson K
中科院分区：
文献类型：
--
作者：
Lukinavičius G;Blaukopf C;Pershagen E;Schena A;Reymond L;Derivery E;Gonzalez-Gaitan M;D'Este E;Hell SW;Wolfram Gerlich D;Johnsson K
细胞可渗透的DNA染色剂是活细胞成像中常用的标记物。目前用于活细胞成像的DNA染色剂要么是有毒的，需要用蓝光照射，要么与超分辨率显微镜不兼容，从而限制了它们的实用性。在这里，我们描述了一种远红DNA染色，SiR-Hoechst，它显示出最小的毒性，适用于不同的细胞类型和组织，并与超分辨率显微镜兼容。这些特性的结合使这种探针成为活细胞成像的强大工具。 现有的用于活细胞显微镜的DNA染色剂要么是有毒的，需要用蓝光照射，要么与超分辨率显微镜不兼容。在这里，作者开发了SiRHoechst，一种无毒的远红DNA染色剂，与超分辨率显微镜兼容。
Cell-permeable DNA stains are popular markers in live-cell imaging. Currently used DNA stains for live-cell imaging are either toxic, require illumination with blue light or are not compatible with super-resolution microscopy, thereby limiting their utility. Here we describe a far-red DNA stain, SiR–Hoechst, which displays minimal toxicity, is applicable in different cell types and tissues, and is compatible with super-resolution microscopy. The combination of these properties makes this probe a powerful tool for live-cell imaging. Existing DNA stains for live cell microscopy are either toxic, require illumination with blue light, or are not compatible with super-resolution microscopy. Here the authors develop SiRHoechst, a non-toxic far-red DNA stain that is compatible with super-resolution microscopy.