Evidence that chlorophyll f functions solely as an antenna pigment in far-red-light photosystem I from Fischerella thermalis PCC 7521
Evidence that chlorophyll f functions solely as an antenna pigment in far-red-light photosystem I from Fischerella thermalis PCC 7521
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DOI：
10.1016/j.bbabio.2020.148184
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发表时间：
2020-06-01
影响因子：
4.3
通讯作者：
Golbeck, John H.
中科院分区：
文献类型：
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作者：
Cherepanov, Dmitry A.;Shelaev, Ivan, V;Golbeck, John H.
蓝藻中的光系统I（PSI）反应中心由类似于96个叶绿素（Chl）分子组成，包括六个专门的Chl分子，称为Chl 1A/Chl 1B（P-700）、Chl 2A/Chl 2B和Chl 3A/Chl 3B，它们排列在两个分支中，并在初级电荷分离中起作用。最近已经提出，来自热色球孢菌（Chroococcidiopsis thermalis）（Nurnberg等人（2018）Science 360，1210-1213）和热费氏菌PCC 7521（Hastings等人（2019）Biochim. Biophys. Acta 1860，452-460）在Chl 2A/Chl 2B位置含有Chl f。我们通过激发来自白光生长（WL-PSI）和远红光生长（FRL-PSI）F的RC来测试该提议。thermalis PCC 7521与飞秒脉冲和分析光学动力学。如果Chl f位于FRL-PSI中的Chl 2A/Chl 2B位置，则在740 nm处的激发应产生电荷分离态P700（+）A（0）（-），随后电子转移到A（1），τ为
The Photosystem I (PSI) reaction center in cyanobacteria is comprised of similar to 96 chlorophyll (Chl) molecules, including six specialized Chl molecules denoted Chl1A/Chl1B (P-700), Chl2A/Chl2B, and Chl3A/Chl3B that are arranged in two branches and function in primary charge separation. It has recently been proposed that PSI from Chroococcidiopsis thermalis (Nurnberg et al. (2018) Science 360, 1210-1213) and Fischerella thermalis PCC 7521 (Hastings et al. (2019) Biochim. Biophys. Acta 1860, 452-460) contain Chl f in the positions Chl2A/Chl2B. We tested this proposal by exciting RCs from white-light grown (WL-PSI) and far-red light grown (FRL-PSI) F. thermalis PCC 7521 with femtosecond pulses and analyzing the optical dynamics. If Chl f were in the position Chl2A/Chl2B in FRL-PSI, excitation at 740 nm should have produced the charge-separated state P700(+)A(0)(-) followed by electron transfer to A(1) with a tau of