Comparison of Diaket and MTA when used as root-end filling materials to support regeneration of the periradicular tissues
Comparison of Diaket and MTA when used as root-end filling materials to support regeneration of the periradicular tissues
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DOI：
10.1046/j.1365-2591.2002.00582.x
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发表时间：
2002-10-01
影响因子：
5
通讯作者：
Witherspoon, DE
中科院分区：
文献类型：
--
作者：
Regan, JD;Gutmann, JL;Witherspoon, DE
目的 本研究的目的是在比较的基础上评估三氧化二矿物质聚集体 (MTA) 和 Diaket 作为外科根尖填充材料促进根尖周组织再生的潜力。 方法 对 7 只体重在 15 至 25 公斤之间的狗进行麻醉，然后对其下颌前磨牙的根管进行介入、清洁、成形和封闭。使用 IRM 修复冠状腔。建立到达根端的手术通路，并切除根端并用超声波尖端进行准备。 MTA 或浓稠混合 Diaket 的根端填充物被随机分配到制剂中。将反射的组织重新定位并用 4-0 vicryl 缝合线缝合。术后六十天，处死动物，用10％中性缓冲福尔马林灌注，并以块状切片取出第三和第四前磨牙。将标本脱矿质并以 6 微米的间隔进行切片以进行组织学评估。切片用苏木精和伊红或Gomori一步三色染色剂染色，并在光学显微镜下检查。所有评估均由两名经过校准的考官进行，并根据既定标准进行评分。调整动物块效应后，使用方差分析对原始数据进行统计评估。结果统计评估表明，观察到的组织对两种根端充填材料的再生反应没有统计学差异。结论在非感染牙齿的根尖周手术中，Diaket和MTA用作根尖充填材料时，可以支持根尖周组织几乎完全再生。
Aim The objective of this study was to evaluate on a comparative basis the potential for mineral trioxide aggregate (MTA) and Diaket to promote periradicular tissue regeneration when used as surgical root-end filling materials.Methodology Seven dogs weighing between 15 and 25 kg were anesthetized prior to having the root canals of their mandibular premolars accessed, cleaned, shaped and obturated. Coronal access cavities were restored with IRM. Surgical access to the root ends was established and the root ends were resected and prepared with ultrasonic tips. Root-end fillings of either MTA or thickly mixed Diaket were randomly assigned to the preparations. Reflected tissues were repositioned and sutured with 4-0 vicryl sutures. Sixty days postsurgery, the animals were killed, perfused with 10% neutral buffered formalin and the third and fourth premolars removed in block sections. The specimens were demineralized and sectioned at 6-mum intervals for histological assessment. Sections were stained with either haematoxylin and eosin or Gomori's one step trichrome stain and examined under the light microscope. All evaluations were made by two calibrated examiners and gradings were scored based on established criteria. The raw data was evaluated statistically using anova after adjusting for the animal block effect.Results Statistical evaluation indicated that there were no statistical differences between the observed regenerative responses of the tissues to the two root-end filling materials.Conclusions Both Diaket and MTA can support almost complete regeneration of the periradicular periodontium when used as root-end filling materials in periradicular surgery on noninfected teeth.