Protoplasmic Changes in Cambial Cells Induced by a Tracheid-Differentiation Factor from Pine Needles

Protoplasmic Changes in Cambial Cells Induced by a Tracheid-Differentiation Factor from Pine Needles
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DOI:
10.1093/jxb/44.2.395
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发表时间:
1993-02
影响因子:
6.9
通讯作者:
R. Savidge;J. Barnett
R. Savidge;J. Barnett
中科院分区:
生物学1区
文献类型:
--
作者:
R. Savidge;J. Barnett

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松树茎插条中“休眠”梭形形成层细胞(fccs)直接分化为管胞。通过光学和透射电子显微镜进行了研究。在除一对针之外的所有插条中,分化发生在靠近针痕迹的地方,而没有事先的细胞分裂或扩增。分化的 fcc 的高嗜锇性颗粒细胞质表现出大量线粒体、网状体和内质网 (ER) 池,并富含囊泡和多核糖体。直到分化的管胞接近成熟时,原生质才像正常情况那样变得高度空泡化
Direct differentiation of «dormant» fusiform cambial cells (fccs) into tracheids in stem cuttings of Pinus contorta Dougl. was investigated by light and transmission electron microscopy. In cuttings from which all but one pair of needles had been removed, differentiation occurred close to the needle trace without prior cell division or expansion. The highly osmiophillic, granular cytoplasm of differentiating fccs exhibited numerous mitochondria, dictyosomes and endoplasmic reticulum (ER) cisternae and was enriched with vesicles and polyribosomes. The protoplasm did not become highly vacuolated, as it normally does, until differentiating tracheids were approaching maturation