THE CELL-DIVISION CYCLE OF TRYPANOSOMA-BRUCEI-BRUCEI - TIMING OF EVENT MARKERS AND CYTOSKELETAL MODULATIONS
THE CELL-DIVISION CYCLE OF TRYPANOSOMA-BRUCEI-BRUCEI - TIMING OF EVENT MARKERS AND CYTOSKELETAL MODULATIONS
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DOI：
10.1098/rstb.1989.0037
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发表时间：
1989-06-12
影响因子：
6.3
通讯作者：
GULL, K
中科院分区：
文献类型：
--
作者：
SHERWIN, T;GULL, K
我们分析了布氏锥虫细胞分裂周期内发生的事件的时间和顺序。处于细胞周期最早阶段的细胞拥有三个主要细胞器的单一副本：细胞核、动质体和鞭毛。细胞周期进展的第一个迹象是位于鞭毛周围的成熟基体附近的前基体的伸长。当新的子鞭毛开始生长时，这个新伸长的基体占据细胞内的后部位置。只有在新的子鞭毛开始生长后，才会产生两个新的前基体。新鞭毛与副鞭毛杆一起延伸，持续贯穿细胞周期的主要部分。在鞭毛伸长的这段时期，发生动质体分裂，两个动质体与两个鞭毛基体一起在细胞内分开。然后启动有丝分裂，并实现细胞器位置的复杂模式，由此分裂平面纵向穿过细胞，使得每个子体最终接收单个核、动质体和鞭毛。这些事件是通过透射电子显微镜对整个细胞骨架的观察以及通过荧光显微镜对特定细胞器的检测来描述的。细胞周期内事件的顺序和时间来源于对指数生长培养物中给定细胞类型比例的分析。
We have analysed the timing and order of events occurring within the cell division cycle of Trypanosoma brucei. Cells in the earliest stages of the cell cycle possess a single copy of three major organelles: the nucleus, the kinetoplast and the flagellum. The first indication of progress through the cell cycle is the elongation of the pro-basal body lying adjacent to the mature basal body subtending the flagellum. This newly elongated basal body occupies a posterior position within the cell when it initiates growth of the new daughter flagellum. Genesis of two new pro-basal bodies occurs only after growth of the new daughter flagellum has been initiated. Extension of the new flagellum, together with the paraflagellar rod, then continues throughout a major portion of the cell cycle. During this period of flagellum elongation, kinetoplast division occurs and the two kinetoplasts, together with the two flagellar basal bodies, then move apart within the cell. Mitosis is then initiated and a complex pattern of organelle positions is achieved whereby a division plane runs longitudinally through the cell such that each daughter ultimately receives a single nucleus, kinetoplast and flagellum. These events have been described from observations of whole cytoskeletons by transmission electron microscopy together with detection of particular organelles by fluorescence microscopy. The order and timing of events within the cell cycle has been derived from analyses of the proportion of a given cell type occurring within an exponentially growing culture.