An approach to the tertiary structure of globular proteins.
An approach to the tertiary structure of globular proteins.
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球状蛋白三级结构的一种方法。
DOI：
10.1021/ja00848a038
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发表时间：
1975
影响因子：
15
通讯作者：
I. Kuntz
中科院分区：
文献类型：
--
作者：
I. Kuntz
结论本文描述的实验证明寡肽和多肽在掺入聚氧乙烯拉伸膜时部分定向。此外，POE 载体的红外特性允许测定 v (NH)、酰胺 I 和 II 跃迁以及低于 800 cm-1 的红外波段的二向色光谱。由于 POE 薄膜能快速吸收大量的水蒸气，因此可以很容易地研究悬浮分子的氢到氘的交换反应。红外二色性和氢交换动力学的结合可以获得重要的新构象信息。
ConclusionsThe experiments described here demonstrate that both oligopeptides and polypeptides are partially oriented upon incorporation in stretched films of polyoxyethylene. Furthermore, the infrared characteristics of the POE support allow determination of dichroic spectra for the v (NH), amide I, and II transitions as well as for infrared bands below~ 800 cm-1. Since POE films rapidly absorb substan-tial amounts of water vapor, hydrogen to deuterium ex-change reactions of suspended molecules can readily be studied. Important new conformational information is available from the combination of infrared dichroism and hydrogen exchange kinetics.