Plasmodium malariae and P. ovale genomes provide insights into malaria parasite evolution.
Plasmodium malariae and P. ovale genomes provide insights into malaria parasite evolution.
复制标题
DOI：
10.1038/nature21038
复制
发表时间：
2017-02-02
期刊：
影响因子：
64.8
通讯作者：
Otto TD
中科院分区：
文献类型：
--
作者：
Rutledge GG;Böhme U;Sanders M;Reid AJ;Cotton JA;Maiga-Ascofare O;Djimdé AA;Apinjoh TO;Amenga-Etego L;Manske M;Barnwell JW;Renaud F;Ollomo B;Prugnolle F;Anstey NM;Auburn S;Price RN;McCarthy JS;Kwiatkowski DP;Newbold CI;Berriman M;Otto TD
由于缺乏三种感染人类的疟原虫物种（P. malariae 和两种 P. ovale 物种（P. o. curtisi 和 P. o. wallikeri））的遗传信息，对感染人类的疟原虫物种的进化历史和相互关系的阐明受到了阻碍。这些物种在疟疾流行的大多数地区普遍存在，并且通常无法通过光学显微镜检测到，这使得它们在人群中的研究变得困难。这些物种与其他感染人类的物种的确切进化关系一直存在争议。使用 P. malariae 的新参考基因组和手动策划的 P. o. 草稿。 curtisi 基因组，我们现在能够准确地将这些物种置于疟原虫系统发育中。对感染黑猩猩的三日疟原虫近缘种的测序揭示了三日疟原虫谱系与另一种疟原虫谱系相似的选择特征，该谱系被证明能够定植于人类和黑猩猩宿主。分子测年表明，这些宿主适应发生在相似的进化时间尺度上。除了物种之间保守的核心基因组之外，基因内容的差异还可以与其特定的生物学联系起来。基因组表明，三日疟原虫在其表面表达一个异二聚体蛋白家族，这些蛋白与对红细胞侵袭至关重要的蛋白具有结构相似性。这里提供的数据提供了对疟原虫属整体进化的深入了解。
Elucidation of the evolutionary history and interrelatedness of Plasmodium species that infect humans has been hampered by a lack of genetic information for three human-infective species: P. malariae and two P. ovale species (P. o. curtisi and P. o. wallikeri). These species are prevalent across most regions in which malaria is endemic and are often undetectable by light microscopy, rendering their study in human populations difficult. The exact evolutionary relationship of these species to the other human-infective species has been contested. Using a new reference genome for P. malariae and a manually curated draft P. o. curtisi genome, we are now able to accurately place these species within the Plasmodium phylogeny. Sequencing of a P. malariae relative that infects chimpanzees reveals similar signatures of selection in the P. malariae lineage to another Plasmodium lineage shown to be capable of colonization of both human and chimpanzee hosts. Molecular dating suggests that these host adaptations occurred over similar evolutionary timescales. In addition to the core genome that is conserved between species, differences in gene content can be linked to their specific biology. The genome suggests that P. malariae expresses a family of heterodimeric proteins on its surface that have structural similarities to a protein crucial for invasion of red blood cells. The data presented here provide insight into the evolution of the Plasmodium genus as a whole.