Microbiota of the indoor environment: a meta-analysis.
Microbiota of the indoor environment: a meta-analysis.
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DOI：
10.1186/s40168-015-0108-3
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发表时间：
2015-10-13
期刊：
影响因子：
15.5
通讯作者：
Meadow JF
中科院分区：
文献类型：
--
作者：
Adams RI;Bateman AC;Bik HM;Meadow JF
作为现代人，我们大部分时间都在室内环境中度过。因此，环境中接触微生物对人类健康具有重要影响，更好地了解影响室内微生物聚集的生态驱动因素和过程将是扩大我们对建筑环境知识的关键。在目前的调查中，我们结合了最近的研究，研究建筑环境中的微生物群，以确定统一的社区模式和室内环境因素的相对重要性。最终，由于室内环境中高通量真菌研究数量有限，本荟萃分析集中于细菌和古菌的研究。我们结合了来自全球16项室内环境调查的16 S核糖体RNA（rRNA）基因数据集，此外还包括7项代表微生物分类群（室外空气，土壤和人体）的推定环境来源的其他研究。共享特定的实验协议或室内栖息地的研究子集的综合分析显示一致的源环境和环境过滤的社区模式指示。此外，我们能够确定室内微生物的几个一致来源，特别是室外空气和皮肤，反映了个别研究中所显示的情况。跨研究的技术差异有很大的影响，微生物群落组合的比较，在实验方案的差异限制了我们的能力，广泛探索的重要性，例如，采样地点，建筑功能和用途，或环境基板在构建室内微生物群落。我们提出了一个重要的科学领域在其早期阶段，研究往往集中在几个地理区域的重采样的快照。从实践的角度来看，这一奋进加强了微生物组研究中阴性“试剂盒”对照的重要性。从了解建筑环境中的机械过程的角度来看，这项荟萃分析证实了地理和建筑类型等广泛因素对室内微生物的结构有影响。然而，这项工作表明，采用常见采样技术的个体研究可能更适合探索微妙的室内环境因素对室内微生物组的相对重要性。本文的在线版本（doi：10.1186/s40168-015-0108-3）包含补充材料，可供授权用户使用。
As modern humans, we spend the majority of our time in indoor environments. Consequently, environmental exposure to microorganisms has important implications for human health, and a better understanding of the ecological drivers and processes that impact indoor microbial assemblages will be key for expanding our knowledge of the built environment. In the present investigation, we combined recent studies examining the microbiota of the built environment in order to identify unifying community patterns and the relative importance of indoor environmental factors. Ultimately, the present meta-analysis focused on studies of bacteria and archaea due to the limited number of high-throughput fungal studies from the indoor environment. We combined 16S ribosomal RNA (rRNA) gene datasets from 16 surveys of indoor environments conducted worldwide, additionally including 7 other studies representing putative environmental sources of microbial taxa (outdoor air, soil, and the human body). Combined analysis of subsets of studies that shared specific experimental protocols or indoor habitats revealed community patterns indicative of consistent source environments and environmental filtering. Additionally, we were able to identify several consistent sources for indoor microorganisms, particularly outdoor air and skin, mirroring what has been shown in individual studies. Technical variation across studies had a strong effect on comparisons of microbial community assemblages, with differences in experimental protocols limiting our ability to extensively explore the importance of, for example, sampling locality, building function and use, or environmental substrate in structuring indoor microbial communities. We present a snapshot of an important scientific field in its early stages, where studies have tended to focus on heavy sampling in a few geographic areas. From the practical perspective, this endeavor reinforces the importance of negative “kit” controls in microbiome studies. From the perspective of understanding mechanistic processes in the built environment, this meta-analysis confirms that broad factors, such as geography and building type, structure indoor microbes. However, this exercise suggests that individual studies with common sampling techniques may be more appropriate to explore the relative importance of subtle indoor environmental factors on the indoor microbiome. The online version of this article (doi:10.1186/s40168-015-0108-3) contains supplementary material, which is available to authorized users.
影响因子：
1.8
作者：
Hwang JM;Lee JH;Yeh JY
通讯作者：
Yeh JY