Expression and clinical significance of stem cell marker CD133 in human neuroblastoma
Expression and clinical significance of stem cell marker CD133 in human neuroblastoma
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DOI：
10.1007/s12519-008-0012-z
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发表时间：
2008-07
影响因子：
8.7
通讯作者：
Q. Tong;Li-duan Zheng;S. Tang;Qing-lan Ruan;Yuan Liu;Shi-wang Li;Guo-song Jiang;Jia-bin Cai
中科院分区：
文献类型：
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作者：
Q. Tong;Li-duan Zheng;S. Tang;Qing-lan Ruan;Yuan Liu;Shi-wang Li;Guo-song Jiang;Jia-bin Cai
背景近年来的研究表明，CD133是一种跨膜蛋白，可以作为神经嵴肿瘤干细胞分离的标志物。本研究旨在探讨干细胞标志物CD133在神经母细胞瘤（NB）中的表达及其临床意义。方法采用免疫组化染色法检测32例NB和8例神经节神经母细胞瘤（GNB）中CD133的表达。分析CD133表达与国际神经母细胞瘤分期系统（INSS）分期、病理分型及NB患者术后生存时间的关系。结果CD133在NB和GNB中的表达率分别为46.9%（15/32）和37.5%(3/8)，主要表达于神经母细胞瘤细胞的细胞质中。1-2期、3-4期和4S期的表达率分别为30.7%、57.9%和37.5%。各阶段差异均有统计学意义（P<0.05）。组织学不良组CD133阳性率（52.4%）显著高于组织学良好组（36.8%），差异有统计学意义（P=0.007）。CD133阴性患者的生存时间明显长于CD133阳性患者（P=0.026）。结论scd133可能与NB的发生、发展有关，可作为判断NB预后的重要指标之一。
BackgroundRecent evidences indicate that CD133, a kind of transmembrane protein, can be used as a marker to isolate stem cells from tumors originating from neural crest. This study was undertaken to explore the expression and clinical significance of stem cell marker CD133 in neuroblastoma (NB).MethodsImmunohistochemical staining was used to detect the expression of CD133 in 32 patients with NB and 8 patients with ganglioneuroblastoma (GNB). The relationships were analyzed among CD133 expression, international neuroblastoma staging system (INSS) stages, pathological classification, and postoperative survival time of NB patients.ResultsThe expression rates of CD133 in NB and GNB were 46.9% (15/32) and 37.5% (3/8) respectively, mainly in cytoplasm of neuroblastoma cells. The expression rates of stage 1–2, stage 3–4 and stage 4S were 30.7%, 57.9% and 37.5%, respectively. The differences in various stages were significant (P<0.05). The positive rate of CD133 in patients with unfavorable histology (52.4%) was significantly higher than that in patients with favorable histology (36.8%) (P=0.007). The survival time of CD133 negative patients was significantly longer than that of CD133 positive patients (P=0.026).ConclusionsCD133 which might be correlated with the development and progression of NB can serve as one of the important indicators for prognosis of NB.