miR-151a-5p promotes the proliferation and metastasis of colorectal carcinoma cells by targeting AGMAT
miR-151a-5p promotes the proliferation and metastasis of colorectal carcinoma cells by targeting AGMAT
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DOI：
10.3892/or.2023.8487
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发表时间：
2023-03-01
期刊：
影响因子：
4.2
通讯作者：
Zhang, Xingxing
中科院分区：
文献类型：
--
作者：
Xie, Yaya;Zhang, Yue;Zhang, Xingxing
结直肠癌（CRC）是最常见的消化道癌症之一。已有研究表明microRNA（miRs）的异位表达在结直肠癌的发生、发展中起着重要作用。此外，也有人提出，miR-151 a-5 p可以作为一种有用的生物标志物，用于早期检测和治疗不同类型的癌症，特别是CRC。然而，miR-151 a-5 p在CRC中的具体作用和潜在机制仍然难以捉摸。目前的研究结果表明，miR-151 a-5 p在CRC细胞系和来自CRC患者的临床组织中上调。在功能上，结果显示miR-151 a-5 p显著促进CRC细胞增殖、迁移和侵袭。此外，双荧光素酶报告基因检测证实胍基丁胺酶（AGMAT）是miR-151 a-5 p的直接靶点，并且与miR-151 a-5 p表达正相关。miR-151 a-5 p可促进大肠癌细胞的上皮-间质转化。综上所述，目前的研究结果揭示了一种新的分子机制，表明miR-151 a-5 p/AGMAT轴在CRC的调控中起着至关重要的作用，因此可以被认为是CRC的潜在治疗策略。
Colorectal carcinoma (CRC) is one of the most common types of digestive cancer. It has been reported that the ectopic expression of microRNAs (miRs) plays a critical role in the occurrence and progression of CRC. In addition, it has also been suggested that miR-151a-5p may serve as a useful biomarker for the early detection and treatment of different types of cancer and particularly CRC. However, the specific effects and underlying mechanisms of miR-151a-5p in CRC remain elusive. The results of the current study demonstrated that miR-151a-5p was upregulated in CRC cell lines and clinical tissues derived from patients with CRC. Functionally, the results showed that miR-151a-5p significantly promoted CRC cell proliferation, migration and invasion. Additionally, dual luciferase reporter assays verified that agmatinase (AGMAT) was a direct target of miR-151a-5p and it was positively associated with miR-151a-5p expression. Mechanistically, miR-151a-5p could enhance the epithelial-mesenchymal transition of CRC cells. Taken together, the results of the current study revealed a novel molecular mechanism indicating that the miR-151a-5p/AGMAT axis could serve a crucial role in the regulation of CRC and could therefore be considered as a potential therapeutic strategy for CRC.