Identification and validation of an over-dominant QTL controlling soybean seed weight using populations derived from Glycine max × Glycine soja.
Identification and validation of an over-dominant QTL controlling soybean seed weight using populations derived from Glycine max × Glycine soja.
复制标题
DOI：
10.1111/pbr.12197
复制
发表时间：
2014-10
期刊：
影响因子：
2
通讯作者：
Long Yan;Ying-hui Li;Chunyan Yang;S. Ren;R. Chang;Meng-chen Zhang;L. Qiu
中科院分区：
文献类型：
--
作者：
Long Yan;Ying-hui Li;Chunyan Yang;S. Ren;R. Chang;Meng-chen Zhang;L. Qiu
几十年来，杂种优势或杂种优势已被用于提高几种重要作物的种子产量，它在大豆中具有潜在的应用。超显性数量性状基因座（QTL）的发现，将有助于通过分子标记辅助选择进行大豆杂交育种。本研究以大豆品种‘冀豆12’×大豆品种‘ZYD 2738’和大豆品种‘冀豆9号’为亲本，利用杂交F_2和F_2：3群体，研究了大豆品种‘冀豆12’和大豆品种‘ZYD 2738’杂交F_2和F_2：max）דZYD 2738”，筛选出与粒重相关的超显性QTL。共检测到7个QTL。其中qSWT_13_1定位在第13染色体上，与Satt 114连锁，在两个群体中连续两代表现超显性效应。用‘冀豆12’בZYD 2738’衍生的6个亚群体进一步检验了这种超显性效应。在不同地点和年份的6个验证群体中，杂合个体的种子重量是纯合个体的1.1 ~ 1.6倍。因此，qSWT_13_1可能是一个有用的位点，以提高杂交大豆的产量和了解大豆杂种优势的分子机制。
Heterosis, or hybrid vigour, has been used to improve seed yield in several important crops for decades and it has potential applications in soybean. The discovery of over-dominant quantitative trait loci (QTL) underlying yield-related traits, such as seed weight, will facilitate hybrid soybean breeding via marker-assisted selection. In this study, F2 and F2 : 3 populations derived from the crosses of ‘Jidou 12’ (Glycine max) × ‘ZYD2738’ (Glycine soja) and ‘Jidou 9’ (G. max) × ‘ZYD2738’ were used to identify over-dominant QTL associated with seed weight. A total of seven QTL were identified. Among them, qSWT_13_1, mapped on chromosome 13 and linked with Satt114, showed an over-dominant effect in two populations for two successive generations. This over-dominant effect was further examined by six subpopulations derived from ‘Jidou12’ × ‘ZYD2738’. The seed weight for heterozygous individuals was 1.1- to 1.6-fold higher than that of homozygous individuals among the six validation populations examined in different locations and years. Therefore, qSWT_13_1 may be a useful locus to improve the yield of hybrid soybean and to understand the molecular mechanism of heterosis in soybean.