Lights, Camera, Action! Antimicrobial Peptide Mechanisms Imaged in Space and Time.
Lights, Camera, Action! Antimicrobial Peptide Mechanisms Imaged in Space and Time.
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DOI：
10.1016/j.tim.2015.11.004
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发表时间：
2016-02
影响因子：
15.9
通讯作者：
Weisshaar JC
中科院分区：
文献类型：
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作者：
Choi H;Rangarajan N;Weisshaar JC
深入了解抗菌肽的抑菌和杀菌机制有助于设计新的抗菌剂。几十年来，各种生物化学测定已应用于大量细菌培养物。虽然这些批量测定中的一些提供1分钟量级的时间分辨率，但它们不能捕获更快的机械事件。它们也不能提供亚细胞空间信息或辨别细菌群体内的细胞与细胞异质性。单细胞时间分辨成像分析为AMP机制研究带来了全新的时空维度。我们回顾了最近的工作，提供了新的见解的时间，序列和空间分布的AMP诱导的细菌细胞的影响。
Deeper understanding of the bacteriostatic and bactericidal mechanisms of antimicrobial peptides (AMPs) should help in the design of new antibacterial agents. Over several decades, a variety of biochemical assays have been applied to bulk bacterial cultures. While some of these bulk assays provide time resolution on the order of 1 min, they do not capture faster mechanistic events. Nor can they provide subcellular spatial information or discern cell-to-cell heterogeneity within the bacterial population. Single-cell, time-resolved imaging assays bring a completely new spatiotemporal dimension to AMP mechanistic studies. We review recent work that provides new insights into the timing, sequence, and spatial distribution of AMP-induced effects on bacterial cells.