Bioleaching of heavy metals from a contaminated soil using indigenous Penicillium chrysogenum strain F1
Bioleaching of heavy metals from a contaminated soil using indigenous Penicillium chrysogenum strain F1
复制标题
使用本地产黄青霉菌株 F1 从受污染的土壤中生物浸出重金属
DOI：
10.1016/j.jhazmat.2012.06.054
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发表时间：
2012-09-30
影响因子：
13.6
通讯作者：
Yuan, Pingfu
中科院分区：
文献类型：
--
作者：
Deng, Xinhui;Chai, Liyuan;Yuan, Pingfu
研究了产黄青霉F1菌株对重金属污染土壤的生物淋滤作用。通过批量实验比较一步法和两步法重金属的浸出效率，并确定生物浸出前后重金属组分的转化情况。结果表明，两步法对重金属的浸出效果优于一步法。当土壤与含产黄青霉F1菌株的培养基质量比为5%（w/v）时，一步去除Cd、Cu、Pb和Zn的效果分别为50%、35%、9%和40%。与一步法相比，两步法对Cd、Cu、Pb和Zn的去除率分别为63%、56%、14%和54%。连续提取结果表明，生物淋滤后土壤中的重金属主要以稳定态存在。透射电镜和扫描电镜观察结果表明，在浸矿过程中，虽然产黄青霉菌丝体破碎成碎片，但活细胞表面没有明显损伤，细胞壁变薄，空泡变小，细胞质浓缩。结果表明，产黄青霉F1菌株可用于去除污染土壤中的重金属。(C)2012爱思唯尔有限公司版权所有。
Bioleaching of heavy metals from contaminated soil using Penicillium chrysogenum strain F1 was investigated. Batch experiments were performed to compare leaching efficiencies of heavy metals between one-step and two-step processes and to determine the transformation of heavy metal fractions before and after bioleaching. The results showed that two-step process had higher leaching efficiencies of heavy metals than one-step process. When the mass ratio of soil to culture medium containing P. chrysogenum strain F1 was 5% (w/v), 50%, 35%, 9% and 40% of Cd, Cu, Pb and Zn were removed in one-step process, respectively. The two-step process had higher removals of 63% Cd, 56% Cu, 14% Pb and 54% Zn as compared with one-step process. The results of the sequential extraction showed that the metals remaining in the soil were mainly bonded in stable fractions after bioleaching. The results of TEM and SEM showed that during bioleaching process, although the mycelium of P. chrysogenum was broken into fragments, no damage was obviously observed on the surface of the living cell except for thinner cell wall, smaller vacuoles and concentrated cytoplasm. The result implied that P. chrysogenum strain F1 can be used to remove heavy metals from polluted soil. (C) 2012 Elsevier B.V. All rights reserved.