Self-assembly of heme A and heme B in a designed four-helix bundle:: Implications for a cytochrome c oxidase maquette
Self-assembly of heme A and heme B in a designed four-helix bundle:: Implications for a cytochrome c oxidase maquette
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DOI：
10.1021/bi000925r
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发表时间：
2000-09-12
期刊：
影响因子：
2.9
通讯作者：
Dutton, PL
中科院分区：
文献类型：
--
作者：
Gibney, BR;Isogai, Y;Dutton, PL
血红素A是细胞色素c氧化酶的辅基[EC 1.9.3.1]，已被引入两种从头设计的四螺旋束蛋白[H10 A2 - 4]（2）和[H10 H2 - 4]（2）中，已知它们分别结合2-4当量的血红素B [Robertson，D. E、法里德河，巴西-地美国，莫泽角C.的方法，Mulholland，S. E、皮迪基蒂河李尔王，J.D.，Wand，A.，J.，DeGrado，W. F.、和达顿，P. L.（1994）Nature 368，425-432]。[H10 A24]（2），{[Ac-CGGGGELWKL.HEELLKK.FEELLKL.AEERLKK. L-CONH 2]（2）}（2），通过在10，10 '位置的双组氨酸连接结合每个四螺旋单元的两个血红素A分子，测量的K-d值为
Heme A, a prosthetic group of cytochrome c oxidase [EC 1.9.3.1], has been introduced into two de novo designed four helix bundle proteins, [H10A24](2) and [H10H24](2), known to bind 2-4 equiv of heme B, respectively [Robertson, D. E., Farid, R. S., Moser, C. C., Mulholland, S. E., Pidikiti, R., Lear, J. D., Wand, A., J., DeGrado, W. F., and Dutton, P. L. (1994) Nature 368, 425-432]. [H10A24](2), {[Ac-CGGGELWKL.HEELLKK.FEELLKL.AEERLKK.L-CONH2](2)}(2), binds two heme A molecules per four-helix unit via bis-histidine Ligation at the 10,10' positions with measured K-d values of