Fused and overlapping rpoB and rpoC genes in Helicobacters, Campylobacters, and related bacteria.
Fused and overlapping rpoB and rpoC genes in Helicobacters, Campylobacters, and related bacteria.
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螺杆菌、弯曲杆菌和相关细菌中融合且重叠的 rpoB 和 rpoC 基因。
DOI：
10.1128/jb.181.12.3857-3859.1999
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发表时间：
1999
影响因子：
3.2
通讯作者：
Severinov,KV
中科院分区：
文献类型：
--
作者：
Zakharova,N;Paster,BJ;Wesley,I;Dewhirst,FE;Berg,DE;Severinov,KV
编码RNA聚合酶β (rpoB)和β′（rpoC）亚基的基因在胃病原体幽门螺杆菌中融合，但在其他分类类群中分离。为了更好地理解这种独特的融合结构是如何进化的，我们测定了10种胃和非胃的幽门杆菌以及相关属（olinella,Arcobacter,Sulfurospirillum, campylobacter）成员的pob - rpocc连接处和周围的DNA序列。我们发现这种融合只存在于幽门螺杆菌和wolinellagenera；另一类的rpoBandrpoCoverlap。这种融合可能是由rpobandrpocoverlap位点的移码突变引起的。好的Shine-Dalgarno序列的缺失可能修复了幽门杆菌科的融合，即使融合本身并没有带来选择优势。
The genes coding for the β (rpoB) and β′ (rpoC) subunits of RNA polymerase are fused in the gastric pathogenHelicobacter pyloribut separate in other taxonomic groups. To better understand how the unique fused structure evolved, we determined DNA sequences at and around therpoB-rpoCjunction in 10 gastric and nongastric species ofHelicobacterand in members of the related generaWolinella,Arcobacter,Sulfurospirillum, andCampylobacter. We found the fusion to be specific toHelicobacterandWolinellagenera;rpoBandrpoCoverlap in the other genera. The fusion may have arisen by a frameshift mutation at the site ofrpoBandrpoCoverlap. Loss of good Shine-Dalgarno sequences might then have fixed the fusion in theHelicobacteraceae, even if fusion itself did not confer a selective advantage.