In vitro maturation of "biotube" vascular grafts induced by a 2-day pulsatile flow loading.
In vitro maturation of "biotube" vascular grafts induced by a 2-day pulsatile flow loading.
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DOI：
10.1002/jbm.b.31405
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发表时间：
2009-10
期刊：
影响因子：
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通讯作者：
Haiying Huang;Yue-Min Zhou;H. Ishibashi-Ueda;K. Takamizawa;J. Ando;K. Kanda;H. Yaku;Y. Nakayama
中科院分区：
文献类型：
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作者：
Haiying Huang;Yue-Min Zhou;H. Ishibashi-Ueda;K. Takamizawa;J. Ando;K. Kanda;H. Yaku;Y. Nakayama
具有小直径的自体血管组织，“生物管”，是使用再生医学中的新概念“体内组织结构技术”在体内开发的。“研究了体外脉动流对生物管结构和功能特性的影响。将用作模具的硅胶棒（直径3.0 mm;长度35.0 mm）嵌入Wister大鼠的背部皮下空间。4周后，收获在杆周围形成的自体管状组织。将一些组织在模拟脉动流的条件下在小口径人动脉中孵育2天（壁面剪切应力（WSS），15.5-77.3 dyn/cm（2）;周向应力（CS），0.6-4.5 x 10（5）dyn/cm（2））。在流动负载时，稀疏的，随机取向的胶原纤维在生物管变得致密，并在规则的圆周方向取向。对照品的符合性（β值）（约30)和流量加载（ca. 20)生物管分别相当于人冠状动脉和股动脉的生物管。此外，在流量加载时，爆破压力从约1000 kPa显著增加。1000 mmHg至约1800 mmHg，沿着α-SMA阳性细胞比率。体外脉动流加载2天可诱导生物管在胶原结构和力学性能方面成熟。
Autologous vascular tissues with a small diameter, "biotubes," were developed in vivo using a novel concept in regenerative medicine, "in-body tissue architecture technology." The effect of pulsatile flow in vitro was investigated on the structural and functional properties of the biotubes. Silicone rods (diameter, 3.0 mm; length, 35.0 mm), used as molds, were embedded into dorsal subcutaneous spaces of Wister rats. After 4 weeks, the autologous tubular tissues formed around the rods were harvested. Some tissues were incubated for 2 days under pulsatile flow simulating conditions in the human arteries with small caliber (wall shear stress (WSS), 15.5-77.3 dyn/cm(2); circumferential stress (CS), 0.6-4.5 x 10(5) dyn/cm(2)). Upon flow loading, the sparse, randomly oriented collagen fibers in the biotubes became dense and oriented in the regular circumferential direction. Compliances (beta values) of the control (ca. 30) and flow-loaded (ca. 20) biotubes were equivalent to that of the human coronary arteries and femoral arteries, respectively. Further, upon flow loading, the burst pressure significantly increased from ca. 1000 mmHg to ca. 1800 mmHg, along with the alpha-SMA-positive cell ratio. Pulsatile flow loading in vitro for 2 days could induce biotube maturation in terms of collagen structures and mechanical properties.