Direct visualization of the oligomeric state of hemagglutinins of influenza virus by high-resolution atomic force microscopy
Direct visualization of the oligomeric state of hemagglutinins of influenza virus by high-resolution atomic force microscopy
复制标题
DOI：
10.1016/j.biochi.2017.12.014
复制
发表时间：
2018-03-01
期刊：
影响因子：
3.9
通讯作者：
Zavyalova, Elena
中科院分区：
文献类型：
--
作者：
Barinov, Nikolay;Ivanov, Nikita;Zavyalova, Elena
高分辨率原子力显微镜（AFM）是一种直接观察单分子的强大技术。在这里，原子力显微镜被应用于表征流感病毒的血凝素的寡聚状态。已知血凝素以三聚体形式存在于病毒包膜内。然而，重组血凝素也作为大的寡聚体存在，这损害了蛋白质的功能活性。用高分辨率原子力显微镜研究了来自病毒株H1、H3、H5、H7和H9的五种商业重组血凝素。与具有功能活性的蛋白质相比，功能失活的血凝素显示出具有更高百分比的大寡聚体。大的低聚物被发现是不稳定的，血凝素的低聚状态受pH值或配体的存在。抗体结合将平衡转移到小的寡聚体，而DNA适体诱导形成大的缔合物。(C)2017 Elsevier B.V.和Societe Francaise de Biochimie et Biologie Moleculaire（SFBBM）。All rights reserved.
High-resolution atomic force microscopy (AFM) is a powerful technique for the direct visualization of single molecules. Here, AFM is applied to characterize the oligomeric state of hemagglutinins of the influenza virus. Hemagglutinins are known to be present in a trimeric form inside the viral envelope. However, recombinant hemagglutinins are also present as large oligomers, which impair the functional activity of the protein. Five commercial recombinant hemagglutinins from the viral strains H1, H3, H5, H7, and H9 were studied with high-resolution AFM. Functionally inactive hemagglutinins were shown to have a higher percentage of large oligomers compared with the proteins with functional activity. Large oligomers were revealed to be unstable; the oligomeric state of hemagglutinin was affected by pH or the presence of ligands. Antibody binding shifts the balance to small oligomers, whereas DNA aptamer induced the formation of large associates. (C) 2017 Elsevier B.V. and Societe Francaise de Biochimie et Biologie Moleculaire (SFBBM). All rights reserved.