CD27/CD70 INTERACTION DIRECTLY DRIVES B-CELL IGG AND IGM SYNTHESIS
CD27/CD70 INTERACTION DIRECTLY DRIVES B-CELL IGG AND IGM SYNTHESIS
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DOI：
10.1002/eji.1830251017
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发表时间：
1995-10-01
影响因子：
5.4
通讯作者：
KOMIYAMA, A
中科院分区：
文献类型：
--
作者：
AGEMATSU, K;KOBATA, T;KOMIYAMA, A
CD 27是在大多数外周血T细胞上表达的T细胞活化抗原。CD 27也在人B细胞亚群上表达，据报道CD 27（+）B细胞分泌IgG和IgM。CD 27的配体CD 70在活化的T和B细胞上表达，表明T和B细胞之间通过CD 27/CD 70连接而相互作用。在这里，我们分析了B细胞免疫球蛋白的合成使用的CD 70转染和目前的功能数据显示，B细胞分泌大量的IgG和IgM的CD 27/CD 70相互作用的结果。流式细胞术分析显示，用金黄色葡萄球菌科万菌株（SAC）加白细胞介素（IL-2）活化后，扁桃体和外周血B细胞上的CD 27表达增加，CD 70表达。另外，加入CD 70转染子可轻度促进B细胞的增殖，抗CD 70单抗可阻断其增殖。更重要的是，在SAC加IL-2的存在下，CD 70转染子极大地增强了纯化的B细胞的IgG和IgM产生。加入抗CD 70 mAb或抗CD 27 mAb可完全阻断这种增强作用。这有力地表明，B细胞上的CD 27与其配体CD 70的相互作用在B细胞生长和分化以产生IgG和IgM中起重要作用。
CD27 is a T cell activation antigen expressed on a majority of peripheral blood T cells. CD27 is also expressed on a subpopulation of human B cells, and it is reported that CD27(+) B cells secrete both IgG and IgM. CD70, a ligand for CD27, is expressed on activated T and B cells, suggesting an interaction between T and B cells via CD27/CD70 ligation. Here, we analyze B cell immunoglobulin synthesis using a CD70 transfectant and present functional data showing that B cells secrete large amounts of IgG and IgM as a result of the CD27/CD70 interaction. A flow cytometric analysis showed that CD27 expression was increased and CD70 was expressed on tonsillar and peripheral blood B cells after activation with Staphylococcus aureus Cowan strain (SAC) plus interleukin (IL-2). In addition, the proliferation of B cells was enhanced mildly by the addition of CD70 transfectant, and its proliferation was blocked by anti-CD70 mAb. More importantly, the CD70 transfectant enhanced IgG and IgM production by purified B cells greatly in the presence of SAC plus IL-2. The enhancement was completely blocked by the addition of either anti-CD70 mAb or anti-CD27 mAb. Strongly suggesting that the interaction of CD27 with its ligand, CD70, on B cells plays an important role in B cell growth and differentiation to produce IgG and IgM.