Identification of a cowpea γ-thionin with bactericidal activity
Identification of a cowpea γ-thionin with bactericidal activity
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DOI：
10.1111/j.1742-4658.2006.05349.x
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发表时间：
2006-08-01
期刊：
影响因子：
5.4
通讯作者：
Bloch, Carlos, Jr.
中科院分区：
文献类型：
--
作者：
Franco, Octavio L.;Murad, Andre M.;Bloch, Carlos, Jr.
抗菌肽是植物界广泛生产的一类丰富的蛋白质化合物。其中，γ-硫宁家族又称为植物防御素，是一个典型的家族，由低分子量富含半胱氨酸的蛋白质组成，通常为阳离子，分布在不同的植物组织中。在这里，我们报告了从豇豆种子（Vigna unguiculata）中纯化和表征的一种新型γ-硫堇，命名为Cp-硫堇II，对革兰氏阳性和革兰氏阴性细菌具有杀菌活性。一旦阐明了一级结构，就可以使用分子模拟实验来研究植物γ-硫堇的多聚化和作用机制。此外，在对比条件下发芽期间，Cp-硫宁 II 也定位于豇豆幼苗的不同组织中，以更好地了解植物保护过程。讨论了植物防御素在构建转基因植物以及生产具有抗人类病原体活性的新药物中的应用。
Antimicrobial peptides are an abundant group of proteinaceous compounds widely produced in the plant kingdom. Among them, the gamma-thionin family, also known as plant defensins, represents one typical family and comprises low molecular mass cysteine-rich proteins, usually cationic and distributed in different plant tissues. Here, we report the purification and characterization of a novel gamma-thionin from cowpea seeds (Vigna unguiculata), named Cp-thionin II, with bactericidal activity against Gram-positive and Gram-negative bacteria. Once the primary structure was elucidated, molecular modelling experiments were used to investigate the multimerization and mechanism of action of plant gamma-thionins. Furthermore, Cp-thionin II was also localized in different tissues in cowpea seedlings during germination in contrasting conditions, to better understand the plant protection processes. The use of plant defensins in the construction of transgenic plants and also in the production of novel drugs with activity against human pathogens is discussed.