Comparison of chemiluminescence enzyme immunoassay based on magnetic microparticles with traditional colorimetric ELISA for the detection of serum α-fetoprotein.
Comparison of chemiluminescence enzyme immunoassay based on magnetic microparticles with traditional colorimetric ELISA for the detection of serum α-fetoprotein.
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DOI：
10.1016/j.jpha.2011.10.001
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发表时间：
2012-04
影响因子：
8.8
通讯作者：
Lin JM
中科院分区：
文献类型：
--
作者：
Zhang QY;Chen H;Lin Z;Lin JM
建立了磁性微粒化学发光酶免疫分析法（MmPs-CLEIA），与传统的比色酶联免疫吸附试验（ELISA）平行检测血清甲胎蛋白（AFP）。系统比较了MmPs-CLEIA和比色ELISA的结果，结果表明，MmPs-CLEIA的免疫试剂用量少，总检测时间短，线性、回收率、精密度、灵敏度和有效性较好。应用MPs-CLEIA和ELISA检测40份血清标本中AFP，并与市售电化学发光免疫分析（ECLIA）试剂盒进行比较。MPs-CLEIA法与ELISA法的相关系数R2=0.6703，但MPs-CLEIA法与ECLIA法的相关性（R2=0.9582）明显优于ELISA法与ECLIA法的相关性（R2=0.6866）。
A chemiluminescence enzyme immunoassay based on magnetic microparticles (MmPs-CLEIA) was developed to evaluate serum α-fetoprotein (AFP) in parallel with traditional colorimetric enzyme-linked immunosorbent assay (ELISA). A systematic comparison between the MmPs-CLEIA and colorimetric ELISA concluded that the MPs-CLEIA exhibited fewer dosages of immunoreagents, less total assay time, and better linearity, recovery, precision, sensitivity and validity. AFP was detected in forty human serum samples by the proposed MPs-CLEIA and ELISA, and the results were compared with commercial electrochemiluminescence immunoassay (ECLIA) kit. The correlation coefficient between MPs-CLEIA and ELISA was obtained with R2=0.6703; however, the correlation between MPs-CLEIA and ECLIA (R2=0.9582) was obviously better than that between colorimetric ELISA and ECLIA (R2=0.6866).