DNA-transformed murine teratocarcinoma cells: regulation of expression of simian virus 40 tumor antigen in stem versus differentiated cells.
DNA-transformed murine teratocarcinoma cells: regulation of expression of simian virus 40 tumor antigen in stem versus differentiated cells.
复制标题
DNA 转化的鼠畸胎癌细胞：干细胞与分化细胞中猿病毒 40 肿瘤抗原表达的调节。
DOI：
10.1073/pnas.77.8.4875
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发表时间：
1980
影响因子：
11.1
通讯作者：
Croce,CM
中科院分区：
文献类型：
--
作者：
Linnenbach,A;Huebner,K;Croce,CM
胸苷激酶缺陷型（TK-；ATP：胸苷 5'-磷酸转移酶，EC 2.7.1.21）F9 畸胎癌干细胞已用重组质粒基因组转化，该基因组由与单纯疱疹病毒 1 型胸苷激酶基因 (HSV-1 tk) 连接的 pBR322 基因组和猿猴病毒 40 组成 （SV40）基因组。获得的干细胞克隆系仅包含一个质粒 DNA 拷贝，该质粒 DNA 通过 pBRR322 基因组上的一个位点整合到小鼠染色体 DNA 中。 HSV-1 tk 基因与 SV40 基因组相邻，在干细胞中表达，而 SV40 基因表达却检测不到。如果诱导这些干细胞的分化，则分化的细胞表达SV40早期基因产物。因此，我们构建了包含一组基因（SV40）的干细胞，其表达在干细胞和分化细胞中受到不同的调节。该细胞系可用于确定干细胞中这些基因表达抑制的机制。
Thymidine kinase-deficient (TK-; ATP:thymidine 5'-phosphotransferase, EC 2.7.1.21)F9 teratocarcinoma stem cells have been transformed with a recombinant plasmid genome consisting of the pBR322 genome linked to a herpes simplex virus type 1 thymidine kinase gene (HSV-1 tk) and a simian virus 40 (SV40) genome. A clonal line of stem cells was obtained that contains only one copy of plasmid DNA, which is integrated into murine chromosomal DNA through a site on the pBRR322 genome. The HSV-1 tk gene, which is adjacent to the SV40 genome, is expressed in stem cells, whereas SV40 gene expression is not detectable. If differentiation of these stem cells is induced, the differentiated cells express SV40 early gene products. Thus, we have constructed a stem cell which contains a set of genes (SV40), the expression of which is regulated differently in stem and differentiated cells. This cell line could be used to determine the mechanism of suppression of expression of these genes in stem cells.