Enhancement of OVA-induced murine lung eosinophilia by co-exposure to contamination levels of LPS in Asian sand dust and heated dust.
Enhancement of OVA-induced murine lung eosinophilia by co-exposure to contamination levels of LPS in Asian sand dust and heated dust.
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DOI：
10.1186/1710-1492-10-30
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
Shibamoto T
中科院分区：
文献类型：
--
作者：
Ren Y;Ichinose T;He M;Song Y;Yoshida Y;Yoshida S;Nishikawa M;Takano H;Sun G;Shibamoto T
先前的研究表明，亚洲沙尘（ASD）对卵清蛋白（OVA）诱导的肺嗜酸性粒细胞增多症的加重在富含脂多糖（LPS）的ASD中比在富含SiO2的ASD中更严重。因此，在本研究中，研究了ASD中不同LPS污染水平对OVA诱导的肺嗜酸性粒细胞增多加重的影响。在开始体内实验之前，我们使用野生型、TLR 2-/-、TLR 4-/-和MyD 88-/- BALB/c小鼠的骨髓来源的巨噬细胞（BMDM）来研究超纯LPS是否仅作用于TLR 4。从沙漠中收集的ASD被加热以去除有毒有机物质（H-ASD）。BALB/c小鼠气管内滴注12种不同的测试样品，所述测试样品用LPS（1 ng和10 ng）、H-ASD和OVA在生理盐水溶液中制备。观察肺组织病理学、支气管肺泡灌洗液（BALF）细胞学、BALF中炎性细胞因子/趋化因子及血清中OVA特异性免疫球蛋白水平。LPS对TLR 4-/-诱导的BMDM产生TNF-α和IL-6无反应，而对TLR 2-/-诱导的BMDM产生TNF-α和IL-6有反应。H-ASD加重LPS诱导的嗜肺细胞炎症反应。在存在OVA的情况下，LPS轻微地增加嗜酸性粒细胞的水平，并且在1 ng和10 ng的水平下诱导痕量水平的Th 2细胞因子IL-5和IL-13。在OVA和H-ASD存在下，LPS诱导气道中严重的嗜酸性粒细胞浸润和杯状细胞增殖，以及BALF中Th 2细胞因子IL-5和IL-13的显著增加。含有LPS（1 ng）的混合物显示出对OVA特异性IgE和IgG 1产生的佐剂活性。结果表明，H-ASD与天然存在的LPS水平，通过增加Th 2介导的细胞因子和抗原特异性免疫球蛋白增强OVA诱导的肺嗜酸性粒细胞增多症。这些结果表明，LPS是ASD中主要加重物质的强有力候选者。
A previous study has shown that the aggravation of Asian sand dust (ASD) on ovalbumin (OVA)-induced lung eosinphilia was more severe in lipopolysaccharide (LPS)-rich ASD than in SiO2-rich ASD. Therefore, the effects of different LPS contamination levels in ASD on the aggravation of OVA-induced lung eosinophilia were investigated in the present study. Before beginning the in vivo experiment, we investigated whether the ultra-pure LPS would act only on TLR4 or not using bone marrow-derived macrophages (BMDMs) of wild–type, TLR2-/-, TLR4-/- and MyD88-/- BALB/c mice. ASD collected from the desert was heated to remove toxic organic substances (H-ASD). BALB/c mice were instilled intratracheally with 12 different testing samples prepared with LPS (1 ng and 10 ng), H-ASD, and OVA in a normal saline solution. The lung pathology, cytological profiles in the bronchoalveolar lavage fluid (BALF), the levels of inflammatory cytokines/chemokines in BALF and OVA-specific immunoglobulin in serum were investigated. The LPS exhibited no response to the production of TNF-α and IL-6 in BMDMs from TLR4-/-, but did from TLR2-/-. H-ASD aggravated the LPS-induced neutrophilic lung inflammation. In the presence of OVA, LPS increased the level of eosinophils slightly and induced trace levels of Th2 cytokines IL-5 and IL-13 at the levels of 1 ng and 10 ng. In the presence of OVA and H-ASD, LPS induced severe eosinophil infiltration and proliferation of goblet cells in the airways as well as remarkable increases in Th2 cytokines IL-5 and IL-13 in BALF. The mixture containing LPS (1 ng) showed adjuvant activity on OVA-specific IgE and IgG1 production. The results suggest that H-ASD with naturally-occurring levels of LPS enhances OVA-induced lung eosinophilia via increases in Th2-mediated cytokines and antigen-specific immunoglobulin. These results indicate that LPS is a strong candidate for being a major aggravating substance in ASD.
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白介素5缺乏消除小鼠哮喘模型中的嗜酸性粒细胞，气道高反应性和肺损伤。
影响因子：
15.3
作者：
Foster, PS;Hogan, SP;Ramsay, AJ;Matthaei, KI;Young, IG
通讯作者：
Young, IG
影响因子：
4.4
作者：
Husar, RB;Tratt, DM;Malm, WC
通讯作者：
Malm, WC
影响因子：
8.3
作者：
Kwon, HJ;Cho, SH;Pershagen, G
通讯作者：
Pershagen, G
DOI：
10.1186/1710-1492-9-19
发表时间：
2013
期刊：
Allergy, asthma, and clinical immunology : official journal of the Canadian Society of Allergy and Clinical Immunology
影响因子：
--
作者：
He M;Ichinose T;Yoshida S;Takano H;Nishikawa M;Sun G;Shibamoto T
通讯作者：
Shibamoto T
影响因子：
4.4
作者：
Dabbagh, K;Dahl, ME;Lewis, DB
通讯作者：
Lewis, DB