Arabinogalactan Protein from a Crude Cell Organelle Fraction of Phaseolus vulgaris L.
Arabinogalactan Protein from a Crude Cell Organelle Fraction of Phaseolus vulgaris L.
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来自菜豆粗细胞器组分的阿拉伯半乳聚糖蛋白。
DOI：
10.1104/pp.68.4.910
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发表时间：
1981
期刊：
影响因子：
7.4
通讯作者：
D. Stegwee
中科院分区：
文献类型：
--
作者：
G. V. van Holst;F. Klis;P. de Wildt;C. Hazenberg;J. Buijs;D. Stegwee
超声处理的粗细胞器馏分从下胚轴组织的黑暗生长的豆幼苗，并从悬浮培养的细胞释放含羟脯氨酸的蛋白质。描述了该蛋白的纯化。发现它是由90%碳水化合物和10%蛋白质组成的阿拉伯半乳聚糖蛋白。主要的糖是半乳糖、阿拉伯糖和糖醛酸，主要的氨基酸是羟脯氨酸、丝氨酸和丙氨酸。其分子量估计为1.4 × 10（5）道尔顿，等电点为pH 2.3。该分子可溶于5%三氯乙酸，可与β-半乳糖基Yariv抗原沉淀。脉冲追踪实验表明该蛋白为分泌性蛋白。阿拉伯半乳聚糖蛋白的生物合成进行了讨论。
Sonication of a crude cell organelle fraction from hypocotyl tissue of dark-grown bean seedlings, and from suspension-cultured cells released a hydroxyproline-containing protein. The purification of this protein is described. It was found to be an arabinogalactan protein composed of 90% carbohydrate and 10% protein. The major sugars are galactose, arabinose, and uronic acids, and the major amino acids are hydroxyproline, serine, and alanine. Its molecular weight was estimated at 1.4 x 10(5) daltons and the isoelectric point at pH 2.3. The molecule is soluble in 5% trichloroacetic acid and can be precipitated with beta-galactosyl Yariv antigen. Pulse-chase experiments indicated that it was a secretory protein. The biosynthesis of arabinogalactan proteins is discussed.