HPLC-MS and Network Pharmacology Analysis to Reveal Quality Markers of Huo-Xue-Jiang-Tang Yin, a Chinese Herbal Medicine for Type 2 Diabetes Mellitus.
HPLC-MS and Network Pharmacology Analysis to Reveal Quality Markers of Huo-Xue-Jiang-Tang Yin, a Chinese Herbal Medicine for Type 2 Diabetes Mellitus.
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HPLC-MS 和网络药理学分析揭示治疗 2 型糖尿病的中草药活血降糖饮的质量标志物
DOI：
10.1155/2021/1072975
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Chen J
中科院分区：
文献类型：
--
作者：
Chen Q;Zhao Y;Li M;Zheng P;Zhang S;Li H;Chen J
Huo-Xue-Jiang-Tang Yin (HXJTY) is a Chinese medicine formulation, which has been widely used for the treatment of various lipometabolism- and glycometabolism-related diseases in clinics. Currently, HXJTY is mainly prescribed to treat patients with type 2 diabetes mellitus (T2DM), yet its chemical and pharmacologic profiles remain to be elucidated. Here, the potential bioactive compound and action mechanism were investigated using chemical and network pharmacology analysis. A rapid HPLC-MS was employed to identify and quantify the component of HXJTY. On the basis of the identified chemical markers from HXJTY, a network pharmacology study, including target gene prediction and functional enrichment, was applied to screen out the main quality markers of HXJTY and explore its potential mechanism for the treatment of T2DM. The results showed that a total of 22 components were identified and quantified from HXJTY by HPLC-MS. Furthermore, 12 active components such as astragaloside IV, calycosin-7-O-β-D-glucoside, hydroxysafflor yellow A, and others were proposed as quality markers of HXJTY for treating T2DM based on network pharmacology analysis. In addition, 125 corresponding possible therapeutic target genes of T2DM were obtained. These target genes are mainly related to peptidase activity, hydrolase activity, phosphatase activity, and cofactor binding, suggesting the involvement of PI3K-Akt, MAPK, AGE-RAGE, and Rap1 signaling pathways in HXJTY-treated T2DM. Our results may provide a useful approach to identify potential quality markers and molecular mechanism of HXJTY for treating T2DM.
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影响因子：
--
作者：
Feng, Tianyu;Li, Kexin;Yao, Yan
通讯作者：
Yao, Yan
DOI：
10.19540/j.cnki.cjcmm.20171010.007
发表时间：
2017-12-01
期刊：
Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica
影响因子：
--
作者：
You, Liang-Zhen;Lin, Yi-Xuan;Wang, Ting-Ting
通讯作者：
Wang, Ting-Ting
网络药理学选择“Q标记”的新策略及其在中药制剂质量控制中的应用
影响因子：
3.6
作者：
Xiang, Wei;Suo, Tong-Chuan;Yu, Hua;Li, An-Ping;Zhang, Shou-Qing;Wang, Chun-Hua;Zhu, Yan;Li, Zheng
通讯作者：
Li, Zheng
DOI：
10.3889/oamjms.2019.165
发表时间：
2019-02-28
影响因子：
--
作者：
Syafril, Santi;Lindarto, Dharma;Mutiara, Erna
通讯作者：
Mutiara, Erna
影响因子：
9.2
作者：
Huang X;Liu G;Guo J;Su Z
通讯作者：
Su Z