Ag2S quantum dot: a bright and biocompatible fluorescent nanoprobe in the second near-infrared window.

Ag2S quantum dot: a bright and biocompatible fluorescent nanoprobe in the second near-infrared window.
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Ag2S 量子点:第二个近红外窗口中的明亮且生物相容性荧光纳米探针。

DOI:
10.1021/nn301218z
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发表时间:
2012-05-22
期刊:
影响因子:
17.1
通讯作者:
Wang Q
Wang Q
中科院分区:
材料科学1区
文献类型:
--
作者:
Zhang Y;Hong G;Zhang Y;Chen G;Li F;Dai H;Wang Q

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在第二近红外区域(NIR-II,1.0~1.4 μm)发射的Ag2S量子点(QD)首次被证明是一种具有明亮光致发光和高生物相容性的有前途的荧光探针。使用具有不同靶向配体的生物相容性 NIR-II Ag2S QD 实现了不同细胞系的高度选择性体外靶向和成像。细胞毒性研究表明,Ag2S QD 在改变细胞增殖、引发细胞凋亡和坏死、产生活性氧 (ROS) 以及引起 DNA 损伤方面的影响可以忽略不计。我们的结果开辟了使用这些生物相容性Ag2S量子点进行体内解剖成像和早期肿瘤诊断的可能性,由于它们在独特的NIR-II成像窗口中的高发射效率,具有深组织穿透性、高灵敏度以及提高的空间和时间分辨率。
Ag2S quantum dots (QDs) emitting in the second near-infrared region (NIR-II, 1.0~1.4 μm) are demonstrated as a promising fluorescent probe with both bright photoluminescence and high biocompatibility for the first time. Highly selective in vitro targeting and imaging of different cell lines are achieved using biocompatible NIR-II Ag2S QDs with different targeting ligands. The cytotoxicity study illustrates the Ag2S QDs with negligible effects in altering cell proliferation, triggering apoptosis and necrosis, generating reactive oxygen species (ROS), and causing DNA damage. Our results have opened up the possibilities of using these biocompatible Ag2S QDs for in vivo anatomical imaging and early-stage tumor diagnosis with deep tissue penetration, high sensitivity, as well as elevated spatial and temporal resolution owing to their high emission efficiency in the unique NIR-II imaging window.
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