Emerging functions of pseudoenzymes.
Emerging functions of pseudoenzymes.
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DOI：
10.1042/bcj20220373
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发表时间：
2023-05-31
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影响因子：
--
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随着序列和结构数据库与强大的分析工具一起沿着增长，假酶的流行和多样性变得越来越明显。假酶存在于生命树的许多酶家族中。假酶被定义为基于序列分析缺乏保守催化基序的蛋白质。然而，一些假酶可能已经迁移了催化所需的氨基酸，使它们能够催化酶促反应。此外，假酶保留了几种非酶的功能，如变构调节，信号整合，支架和竞争性抑制。在这篇综述中，我们提供了使用假激酶，pseudophosphatase，和pseudo ADP-核糖基转移酶家族的每种作用模式的例子。我们强调的方法，促进假酶的生化和功能特性，以鼓励在这个新兴领域的进一步调查。
As sequence and structural databases grow along with powerful analysis tools, the prevalence and diversity of pseudoenzymes have become increasingly evident. Pseudoenzymes are present across the tree of life in a large number of enzyme families. Pseudoenzymes are defined as proteins that lack conserved catalytic motifs based on sequence analysis. However, some pseudoenzymes may have migrated amino acids necessary for catalysis, allowing them to catalyze enzymatic reactions. Furthermore, pseudoenzymes retain several non-enzymatic functions such as allosteric regulation, signal integration, scaffolding, and competitive inhibition. In this review, we provide examples of each mode of action using the pseudokinase, pseudophosphatase, and pseudo ADP-ribosyltransferase families. We highlight the methodologies that facilitate the biochemical and functional characterization of pseudoenzymes to encourage further investigation in this burgeoning field.
影响因子：
16.6
作者：
Stiegler AL;Boggon TJ
通讯作者：
Boggon TJ