Effects of membrane potential on electrical slow waves of canine proximal colon.
Effects of membrane potential on electrical slow waves of canine proximal colon.
复制标题
膜电位对犬近端结肠电慢波的影响。
DOI：
10.1152/ajpcell.1988.255.6.c828
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
Sanders,KM
中科院分区：
文献类型：
--
作者：
Smith,TK;Reed,JB;Sanders,KM
使用犬结肠的环形肌测试膜电位对慢波波形和传播的影响。使用细胞内记录技术对犬近端结肠的横截面条进行研究。粘膜下层附近的圆形肌细胞产生慢波，当它们传播到圆形层时，其幅度会衰减。作为与粘膜下边界的距离的函数，细胞的膜电位的负值较小。使用分区室技术用升高的外部 K+ 和电脉冲对粘膜下层附近的细胞进行去极化。将去极化粘膜下细胞的波形与从大部分圆形层中的细胞记录的事件进行比较。实验去极化引起的波形变化与传播过程中发生的波形变化不同，这表明后者的机制与去极化不同。还评估了膜电位对合胞体输入电阻和长度常数的影响。这些研究的结果与犬近端结肠中的慢波传播被动发生的假设一致。
The effects of membrane potential on the waveforms and propagation of slow waves were tested using circular muscles of the canine colon. Studies were conducted with intracellular recording techniques on cross-sectional strips of canine proximal colon. Circular muscle cells near the submucosa generated slow waves that decayed in amplitude as they spread through the circular layer. The membrane potentials of cells were less negative as a function of distance from the submucosal border. Cells near the submucosa were depolarized with elevated external K+ and electrical pulses using the partitioned chamber technique. The waveforms of depolarized submucosal cells were compared with events recorded from cells in the bulk of the circular layer. The waveform changes caused by experimental depolarization were different from the changes in waveform that occur during propagation, suggesting the latter are due to a different mechanism than depolarization. The effects of the membrane potential on syncytial input resistance and length constant were also evaluated. The results of these studies are consistent with the hypothesis that slow-wave propagation across the circular layer in canine proximal colon occurs passively.