Lysozyme-dextran core-shell nanogels prepared via a green process
Lysozyme-dextran core-shell nanogels prepared via a green process
复制标题
绿色工艺制备溶菌酶-葡聚糖核壳纳米凝胶
DOI：
10.1021/la702785b
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发表时间：
2008-04-01
期刊：
影响因子：
3.9
通讯作者：
Jiang, Ming
中科院分区：
文献类型：
--
作者：
Li, Juan;Yu, Shaoyong;Jiang, Ming
发展了一种制备以溶菌酶为核、葡聚糖为壳的纳米凝胶的新方法。该方法包括美拉德干热法和热凝胶法。首先，通过美拉德反应产生溶菌酶-葡聚糖缀合物。然后，将缀合物溶液加热至高于溶菌酶的变性温度以产生纳米凝胶。纳米凝胶为球形，具有约200 nm的流体动力学直径和约30的溶胀比。纳米凝胶溶液对长期储存以及pH和离子强度的变化是稳定的。ICPs已被用作药物模型，以研究在不同pH值下与这些纳米凝胶的静电和疏水相互作用。研究表明，纳米凝胶更适合于负载质子化布洛芬。我们证实了对溶菌酶-葡聚糖纳米凝胶形成机理的认识可以应用于其他球状蛋白-葡聚糖纳米凝胶的制备。
A novel method has been developed for preparing nanogels with a lysozyme core and dextran shell. The method involves the Maillard dry-heat process and heat-gelation process. First, lysozyme-dextran conjugates were produced through the Maillard reaction. Then, the conjugate solution was heated above the denaturation temperature of lysozyme to produce nanogels. The nanogels are of spherical shape having a hydrodynamic diameter of about 200 nm and swelling ratio of about 30. The nanogel solutions are stable against long-term storage as well as changes in pH and ionic strength. Ibuprofen has been used as a drug model to study the electrostatic and hydrophobic interactions with these nanogels at different pH values. The study reveals that the nanogels are more suitable for loading protonated ibuprofen. We have verified that the knowledge of the formation mechanism of lysozyme-dextran nanogels can be applied to prepare other globular protein-dextran nanogels.