Pentraxin 3 as a new biomarker of peritoneal injury in peritoneal dialysis patients
Pentraxin 3 as a new biomarker of peritoneal injury in peritoneal dialysis patients
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DOI：
10.1007/s10047-012-0663-3
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发表时间：
2013-03
影响因子：
1.3
通讯作者：
Reo Kanda;C. Hamada;K. Kaneko;Takanori Nakano;Keiichi Wakabayashi;H. Io;S. Horikoshi;Y. Tomino
中科院分区：
文献类型：
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作者：
Reo Kanda;C. Hamada;K. Kaneko;Takanori Nakano;Keiichi Wakabayashi;H. Io;S. Horikoshi;Y. Tomino
众所周知，生物不相容性腹膜透析液在腹膜纤维化的发展中起着核心作用。腹膜炎症即使在腹膜透析液刺激停止后仍持续存在。在腹膜透析（PD）停止时，确定腹膜腔持续性炎症的定义非常重要。本研究的目的是确定腹膜流出液（PE）中的五聚蛋白3（PTX 3）是否可能是PD患者的新生物标志物。从顺天堂大学医院的50例终末期肾病患者中获得血清、PE和腹膜标本。19例患者的样本在PD开始时获得，31例患者的样本在PD停止时获得。分析PTX 3、高敏CRP、MMP-2和IL-6。使用抗PTX 3抗体进行免疫组织学检查。在腹膜中的内皮细胞、成纤维细胞和间皮细胞中观察到PTX 3表达。PD停止时PE中的PTX 3水平显著高于PD开始时。有腹膜炎病史或PD持续时间超过8年的患者的流出物PTX 3水平显著高于无腹膜炎或PD持续时间<8年的患者。PTX 3水平与PE中MMP-2、IL-6水平、间皮致密带厚度及血管病变程度显著相关。PTX 3可能是腹膜炎症和进行性纤维化的新生物标志物。
It is well known that bioincompatible peritoneal dialysate plays a central role in the development of peritoneal fibrosis. Peritoneal inflammation continues even after the cessation of peritoneal dialysate stimulation. It is important to establish the definition of persistent inflammation in the peritoneal cavity at the cessation of peritoneal dialysis (PD). The objective of the present study was to determine whether pentraxin 3 (PTX3) in peritoneal effluent (PE) may be a new biomarker in PD patients. Serum, PE, and peritoneal specimens were obtained from 50 patients with end-stage kidney disease at Juntendo University Hospital. Samples of 19 patients were obtained at the initiation of PD and those of 31 patients at the cessation of PD. PTX3, high-sensitivity CRP, and MMP-2 and IL-6 were analyzed. An immunohistological examination using an anti-PTX3 antibody was performed. Expressions of PTX3 were observed in endothelial cells, fibroblasts, and mesothelial cells in the peritoneum. The PTX3 level in PE at the cessation of PD was significantly higher than that at the initiation of PD. Effluent PTX3 levels in patients with a history of peritonitis or a PD duration of more than 8 years were significantly higher than those in patients without peritonitis or patients with a PD duration of <8 years. The PTX3 level was significantly correlated with MMP-2 and IL-6 levels in PE, as well as the thickness of the submesothelial compact zone and the vasculopathy. It appears that PTX3 may be a new biomarker of peritoneal inflammation and progressive fibrosis.