Thermoacoustic molecular imaging of small animals.
Thermoacoustic molecular imaging of small animals.
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DOI：
10.1162/153535003322331993
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发表时间：
2003-04-01
影响因子：
2.8
通讯作者：
Miller, Kathy D
中科院分区：
文献类型：
--
作者：
Kruger, Robert A;Kiser, William L;Miller, Kathy D
我们设计、制造并测试了一种热声计算机断层扫描（TCT）扫描仪，用于对小动物的光学吸收进行三维成像。该装置利用脉冲激光照射（680-1064 nm）和一个独特的128元换能器阵列。我们将该扫描仪的各向同性空间分辨率量化为0.35 mm。我们描述了一种用TCT分离光学染料的双波长减法技术。幻影实验表明，我们可以使用双波长减法在1微升体积中检测到5 fmol的近红外染料（吲哚菁绿，ICG）。在幻影和两只牺牲小鼠中进行的初始TCT成像表明，与荧光成像或扩散光学断层扫描相比，小动物的三维光学吸收模式可以以更好的数量级空间分辨率和更好的数量级低对比度检测小动物。
We have designed, constructed, and tested a thermoacoustic computed tomography (TCT) scanner for imaging optical absorption in small animals in three dimensions. The device utilizes pulsed laser irradiation (680-1064 nm) and a unique, 128-element transducer array. We quantified the isotropic spatial resolution of this scanner to be 0.35 mm. We describe a dual-wavelength subtraction technique for isolating optical dyes with TCT. Phantom experiments demonstrate that we can detect 5 fmol of a near-infrared dye (indocyanine green, ICG) in a 1-microL volume using dual-wavelength subtraction. Initial TCT imaging in phantoms and in two sacrificed mice suggests that three-dimensional, optical absorption patterns in small animals can be detected with an order of magnitude better spatial resolution and an order of magnitude better low-contrast detectability in small animals when compared to fluorescence imaging or diffusion optical tomography.