Functional role of stromal interaction molecule 1 (STIM1) in vascular smooth muscle cells
Functional role of stromal interaction molecule 1 (STIM1) in vascular smooth muscle cells
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DOI：
10.1016/j.bbrc.2007.07.096
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发表时间：
2007-10-05
影响因子：
3.1
通讯作者：
Ito, Hiroshi
中科院分区：
文献类型：
--
作者：
Takahashi, Yoichiro;Watanabe, Hiroyuki;Ito, Hiroshi
我们研究了STIM1在血管平滑肌细胞（VSMCs）中的功能作用，STIM1是内质网（ER）中的一种钙离子传感器，它调节钙库操纵性钙内流（SOCE）。STIM1主要定位于内质网和细胞膜。通过小干扰（si）RNA敲低STIM1的表达会大幅降低SOCE。相反，STIM1的一个EF手结构域突变体STIM1（E87A）使SOCE显著增加，而与瞬时受体电位经典型1（TRPC1）的siRNA共转染可消除这种增加。此外，用针对STIM1的siRNA转染可抑制环磷腺苷效应元件结合蛋白（CREB）的磷酸化和细胞生长。这些结果表明，STIM1是SOCE的一个重要组成部分，并且它参与血管平滑肌细胞的增殖。（C）2007爱思唯尔公司。保留所有权利。
We investigated the functional role of STIM1, a Ca2+ sensor in the endoplasmic reticulum (ER) that regulates store-operated Ca2+ entry (SOCE), in vascular smooth muscle cells (VSMCs). STIM1 was mainly localized at the ER and plasma membrane. The knockdown of STIM1 expression by small interfering (si) RNA drastically decreased SOCE. In contrast, an EF-hand mutant of STIM1, STIM1(E87A), produced a marked increase in SOCE, which was abolished by co-transfection with siRNA to transient receptor potential canonical 1 (TRPC1). In addition, transfection. with siRNA against STIM1 suppressed phosphorylation of cAMP-responsive element binding protein (CREB) and cell growth. These results suggest that STIM1 is an essential component of SOCE and that it is involved in VSMC proliferation. (C) 2007 Elsevier Inc. All rights reserved.