Detection and Size Analysis of Proteins with Switchable DNA Layers
Detection and Size Analysis of Proteins with Switchable DNA Layers
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DOI：
10.1021/nl8026789
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发表时间：
2009-04-01
期刊：
影响因子：
10.8
通讯作者：
Abstreiter, Gerhard
中科院分区：
文献类型：
--
作者：
Rant, Ulrich;Pringsheim, Erika;Abstreiter, Gerhard
我们介绍了一种芯片兼容的方案，用于实时无标记检测蛋白质，该方案基于金属表面上的DNA寡核苷酸的电驱动构象转换。开关行为是特异性识别IgG抗体和抗体片段的灵敏指标，其可以在传感器表面上以小于10（-18）mol的量检测。此外，我们展示了如何通过测量高频开关响应来监测诱导分子运动的动力学。当蛋白质结合到层上时，流体动力学阻力的增加减缓了切换动力学，这使我们能够确定捕获的蛋白质的大小。我们证明了不同的抗体片段的识别，通过他们的动力学指纹。switchDNA方法代表了使用单个分析平台同时检测和确定靶分子大小的通用方法。
We introduce a chip-compatible scheme for the label-free detection of proteins in real-time that is based on the electrically driven conformation switching of DNA oligonucleotides on metal surfaces. The switching behavior is a sensitive indicator for the specific recognition of IgG antibodies and antibody fragments, which can be detected in quantities of less than 10(-18) mol on the sensor surface. Moreover, we show how the dynamics of the induced molecular motion can be monitored by measuring the high-frequency switching response. When proteins bind to the layer, the increase in hydrodynamic drag slows the switching dynamics, which allows us to determine the size of the captured proteins. We demonstrate the identification of different antibody fragments by means of their kinetic fingerprint. The switchDNA method represents a generic approach to simultaneously detect and size target molecules using a single analytical platform.