Glucose sensing electrodes based on a poly(3,4-ethylenedioxythiophene)/Prussian blue bilayer and multi-walled carbon nanotubes
Glucose sensing electrodes based on a poly(3,4-ethylenedioxythiophene)/Prussian blue bilayer and multi-walled carbon nanotubes
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基于聚（3,4-亚乙二氧基噻吩）/普鲁士蓝双层膜和多壁碳纳米管的葡萄糖传感电极
DOI：
10.1016/j.bios.2008.10.010
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发表时间：
2009-03-15
影响因子：
12.6
通讯作者：
Chen, Lin-Chi
中科院分区：
文献类型：
--
作者：
Chiu, Jing-Yang;Yu, Chung-Mu;Chen, Lin-Chi
在这里，我们报告了一种新的葡萄糖传感电极的基础上聚（3，4-乙撑二氧噻吩）（PEDOT）/普鲁士蓝（PB）双层和多壁碳纳米管（CNT）。通过连续电沉积在柔性丝网印刷碳电极（SPCE）上制备双层膜。内PB层首先电沉积用于检测来自葡萄糖氧化的H2 O2;外PEDOT层在烘烤或未烘烤的PB膜上电聚合以捕获葡萄糖氧化酶（GOD）。观察到PB在磷酸盐缓冲盐水（pH 7.4）中的稳定性通过在100 ° C和外部PEDOT层两者下的沉积后烘烤获得。此外，烘烤PB膜增强了随后的PEDOT生长和相应的GOD截留。结果，双层酶电极显示出高度分辨和可重复的信号（R.S.D.= 2.54%）的葡萄糖样品从100 μ M至1 M在流动注射分析（FIA）在-0.1 Vvs。Ag/AgCl。线性范围（1-10 mM）的灵敏度为2.67 μ A cm（-2）mM（-1）。此外，电极保持ca。在pH6.0的PBS中4 ℃保存1个月后，其响应为原始响应的82%，并且可以精确地测定人血清中的葡萄糖水平。此外，发现CNT掺入可以进一步提高灵敏度，并且可以实现μ M范围的葡萄糖检测。(C)2008 Elsevier B. V.保留所有权利。
Here we report a new glucose sensing electrode based on a poly(3,4-ethylenedioxythiophene) (PEDOT)/Prussian blue (PB) bilayer and multi-walled carbon nanotubes (CNT). The bilayer was prepared on a flexible screen-printed carbon electrode (SPCE) by sequential electrodeposition. The inner PB layer was electrodeposited first for detecting H2O2 from glucose oxidation; the outer PEDOT layer was electropolymerized on a baked or an unbaked PB film to entrap glucose oxidase (GOD). It was observed that the stability of PB in phosphate buffered saline (pH 7.4) was attained by post-deposition bake at 100 degrees C and the outer PEDOT layer both. In addition, a baked PB film enhanced the subsequent PEDOT growth and the corresponding GOD entrapment. As a result, the bilayer enzyme electrode showed highly resolved and reproducible signals (R.S.D.=2.54%) to glucose samples from 100 mu M to I M during a flow-injection analysis(FIA)at -0.1 Vvs. Ag/AgCl. The sensitivity of the linear range (1-10 mM) was 2.67 mu A cm(-2) mM(-1). Moreover, the electrode retained ca. 82% of the original response after 1-month storage in PBS, pH 6.0 at 4 degrees C and could determine the glucose level in human serum precisely. Besides, it was found that CNT incorporation could further improve the sensitivity and could achieve mu M-range glucose detection. (C) 2008 Elsevier B.V. All rights reserved.