High-level expression of cyp51B in azole-resistant clinical Aspergillus fumigatus isolates
High-level expression of cyp51B in azole-resistant clinical Aspergillus fumigatus isolates
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DOI：
10.1093/jac/dks451
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发表时间：
2013-03-01
影响因子：
5.2
通讯作者：
Bowyer, P.
中科院分区：
文献类型：
--
作者：
Buied, A.;Moore, C. B.;Bowyer, P.
烟曲霉对唑类抗真菌药物的耐药性不是由靶基因突变介导的，现在在一些地方很常见。本研究的目的是探讨非靶标耐药可能的新的耐药机制。对12株先前未显示携带cyp51A突变的耐唑烟曲霉分离株进行了检测，以确定替代cyp51B基因是否过表达。在12株分离株中，一株暴露于伊曲康唑后cyp51B过度诱导，另一株cyp51B高组成表达。cyp51B过表达可能是烟曲霉耐药的机制。
Resistance to azole antifungal drugs in Aspergillus fumigatus that is not mediated by target gene mutations is now common in some locations. The aim of this study was to investigate possible new mechanisms of resistance in non-target resistance.Twelve azole-resistant A. fumigatus isolates previously shown not to carry mutations in cyp51A were tested to determine whether the alternative cyp51B gene was overexpressed.Of 12 isolates one showed overinduction of cyp51B after exposure to itraconazole and another showed high constitutive expression of cyp51B.cyp51B overexpression is a possible azole resistance mechanism in A. fumigatus.