In vivo assessment of thermal damage in the liver using optical spectroscopy
In vivo assessment of thermal damage in the liver using optical spectroscopy
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DOI：
10.1117/1.1779627
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发表时间：
2004-09-01
影响因子：
3.5
通讯作者：
Lin, WC
中科院分区：
文献类型：
--
作者：
Buttemere, CR;Chari, RS;Lin, WC
对于大多数肝癌患者来说，切除术不是一种可行的治疗选择。切除术的替代方法包括热疗法，如射频消融;然而，这些疗法缺乏关于组织热损伤程度和边缘的充分术中反馈。在这项原理研究的证明中，我们测试了荧光和漫反射光谱法评估体内局部热损伤的能力。使用便携式基于光纤的光谱系统，从经历射频消融的健康犬肝组织中获取体内光谱。在热凝固上观察到的主要光谱变化是在480 nm处的荧光发射峰的红移，在总体荧光强度的降低，以及在450至750 nm的漫反射率的增加。光谱变化进行了量化和相关的组织组织学。我们发现了一个很好的相关性之间的建议光谱相关的热损伤和组织学。这项研究的结果表明，荧光和漫反射光谱显示出强大的潜力，作为工具，监测肝组织热损伤术中。(C)2004年，美国光电仪器工程师学会。
Resection is not a viable treatment option for the majority of liver cancer patients. Alternatives to resection include thermotherapies such as radio-frequency ablation; however, these therapies lack adequate intraoperative feedback regarding the degree and margins of tissue thermal damage. In this proof of principle study, we test the ability of fluorescence and diffuse reflectance spectroscopy to assess local thermal damage in vivo. Spectra were acquired in vivo from healthy canine liver tissue undergoing radio-frequency ablation using a portable fiber-optic-based spectroscopic system. The major observed spectral alterations on thermal coagulation were a red shift in the fluorescence emission peak at 480 nm, a decrease in the overall fluorescence intensity, and an increase in the diffuse reflectance from 450 to 750 nm. Spectral changes were quantified and correlated to tissue histology. We found a good correlation between the proposed spectral correlates of thermal damage and histology. The results of this study suggest that fluorescence and diffuse reflectance spectroscopy show strong potential as tools to monitor liver tissue thermal damage intraoperatively. (C) 2004 Society of Photo-Optical Instrumentation Engineers.