The comparative analysis of serum proteomes for the discovery of biomarkers for acute myeloid leukemia
The comparative analysis of serum proteomes for the discovery of biomarkers for acute myeloid leukemia
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DOI：
10.1016/j.exphem.2004.06.006
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发表时间：
2004-09-01
影响因子：
2.6
通讯作者：
Kwak, YG
中科院分区：
文献类型：
--
作者：
Kwak, JY;Ma, TZ;Kwak, YG
客观的。急性髓系白血病 (AML) 是造血前体细胞发生一系列遗传变化的结果。然而，AML 的明确诊断蛋白生物标志物仍不清楚。我们的研究旨在通过侵入性较小的方法确定用于初步诊断、检测复发和监测 AML 微小残留病的生物标志物，分析了反映蛋白质组变化的血清蛋白。材料和方法。我们比较了 12 名 AML 患者和 12 名正常人血清的二维电泳图谱。通过基质辅助激光解吸/电离飞行时间和电喷雾电离四极杆飞行时间质谱法鉴定差异表达的斑点。结果。发现了 AML 组中差异表达的八种蛋白质。 AML患者血清中α-2-HS-糖蛋白、补体相关蛋白SP-40、40、RBP4基因产物、脂蛋白C-III和未知蛋白表达水平下调，而免疫球蛋白重链变体、蛋白酶体26S ATP酶亚基1和触珠蛋白-1等其他3种蛋白表达水平上调。结论。这些结果表明，如果进行进一步的研究，这些蛋白质可以用作 AML 的侵入性较小的诊断和监测生物标志物。 (C) 2004 年国际实验血液学学会。由爱思唯尔公司出版
Objective. Acute myeloid leukemia (AML) develops as the consequence of a series of genetic changes in a hematopoietic precursor cell. However, the definitive diagnostic protein biomarkers for AML are still unclear. In our study to identify the biomarkers for an initial diagnosis, detection of relapse, and monitoring the minimal residual disease in AML by a less invasive method, serum proteins reflecting alterations in their proteomes were analyzed.Materials and Methods. We compared the two-dimensional electrophoresis patterns of human sera of 12 patients with AML with those of 12 normal subjects. The differentially expressed spots were identified by matrix-assisted laser desorption/ionization time-of-flight and electrospray ionization quadupole time-of-flight mass spectrometries.Results. Eight proteins that expressed differentially in the AML group were found. The expression levels of alpha-2-HS-glycoprotein, complement-associated protein SP-40, 40, RBP4 gene product, lipoprotein C-III, and an unknown protein were downregulated in serum of AML patients, whereas the other three proteins, including immunoglobulin heavy-chain variant, proteosome 26S ATPase subunit 1, and haptoglobin-1 were upregulated.Conclusion. These results suggest that these proteins can be used as less invasive diagnostic and monitoring biomarkers of AML if further studies are done. (C) 2004 International Society for Experimental Hematology. Published by Elsevier Inc.