Inheritance of high oleic acid content in the seed oil of mutant Ethiopian mustard lines and its relationship with erucic acid content
Inheritance of high oleic acid content in the seed oil of mutant Ethiopian mustard lines and its relationship with erucic acid content
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DOI：
10.1017/s0021859607006971
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发表时间：
2007-08-01
影响因子：
2
通讯作者：
De Haro-Bailon, A.
中科院分区：
文献类型：
--
作者：
Del Rio-Celestino, M.;Font, R.;De Haro-Bailon, A.
种子油中油酸(C18：1)含量不同的埃塞俄比亚芥菜(Brassica Carinata)基因型可用于食品和工业用途。本研究的目的是研究埃塞俄比亚芥菜不同品系籽油中高C18：1的遗传及其与芥酸含量(C22：1)的关系。用化学诱变剂处理C-101种子，获得了低C18：1/高C22：I突变株L-180 6、高C18：1/高C22：1突变株L-482、高C18：1/低C22：1突变株L-2 890和低C18：1/极高C22：1突变株L-16 30。高C18：1/零C22：1系由埃塞俄比亚芥菜与油菜籽和印度芥菜种间杂交而成。以L-2890x C-101、L-482x L-2890、L-1630x L-25X-1、L-1630x L-2890和L-482x L-1806为材料，进行正反交，获得F-2代和BC_1F_1代。在所有杂交组合中均未观察到细胞质效应。在所研究的杂交组合中，F2和BC1F1群体的分离模式不同。分离该脂肪酸的杂交组合中C18：1含量的遗传符合1个(L-482xL-1806)、2个(L-2890xC-101)或3个(L-1630x L-2890、L-1630x L-25X-1和L-482x2890)座位的预期遗传。油酸分离表明油酸的积累受到两个分离遗传系统的控制，一个作用于从C18：1到C22：1的链延长，另一个涉及从C18：1到亚油酸(C18：2)的去饱和度。C18：1积累量；和C22：1受相同的基因座(M1、M2、E1和E2)影响，控制着从C18：I到C22：1的链延长步骤。此外，C18：1还受一个额外的控制C18：1脱饱和度的基因座(暂定为OL)影响，形成C18：2。L-2890的亲本的遗传组成为OlE(1)E(1)E(2)E(2)m(1)m(1)m(2)。OLE(1)E(1)E(2)E(2)M(1)M(2)M(2)，OLE(1)E(1)E(2)E(2)M(1)M(2)M(2)，L-25X-1的OLOLE(1)E(1)E(2)E(2)M(1)M(2)M(2)，L-482的OL1OLE(1)E(1)E(2)E(2)M(1)M(2)M(2)和L-1806年的OL1OL1E(1)E(1)E(2)E(2)M(1)M(2)M(2)。从L-1630×L-25X-1杂交组合中获得了转基因重组子，其C18：1含量(~gt；643g/kg)是亲本的3倍，不含C22：I，具有较高的商业开发潜力。
Ethiopian mustard (Brassica carinata) genotypes with different contents of oleic acid (C 18: 1) in the seed oil could be useful for food and industrial applications. The objectives of the present research were to study the inheritance of high C18:1 in the seed oil of different lines of Ethiopian mustard and its relationship with erucic acid content (C22: 1). The low C 18: 1 /high C22: I mutant line L- 1806, the high C18: 1/high C22: 1 mutant line L-482, the high C18: 1/low C22: 1 mutant line L-2890 and the low C18: 1/very high C22: 1 mutant line L-1630 were isolated after a chemical mutagen treatment of C-101 seeds (about 94 g C 18: 1 /kg and 450 g C22: 1/kg). The high C 18: 1/zero C22: 1 line L-25X-1 was obtained by interspecific crosses of Ethiopian mustard with rapeseed and Indian mustard. Plants of lines L-2890 x C- 10 1, L-482 x L-2890, L- 1630 x L-25X- 1, L- 1630 x L-2890 and L-482 x L- 1806 were reciprocally crossed and F-2 and the BC1F1 generations were obtained. Cytoplasmic effects were not observed in any of the crosses. The segregation pattern in F2 and BC1F1 populations differed in the crosses studied. The inheritance of C18:1 content in crosses segregating for this fatty acid was that expected for one (crosses between L-482xL-1806), two (L-2890xC-101) or three (L- 1630 x L-2890, L- 1630 x L-25X- I and L-482 x 2890) loci. Oleic acid segregation indicated control of accumulation by two segregating genetic systems, one acting on chain elongation from C18:1 to C22:1 and the other involving desaturation from C18:1 to linoleic acid (C18:2). Accumulations of C18:1;and C22:1 were influenced by the same loci (M1, M2, E1 and E2), which control the chain elongation steps leading from C18: I to C22:1. In addition, C18:1 was influenced by one additional locus (tentatively named OL) involved in control of desaturation of C18:1 to form C18:2. The genetic constitution of the parent lines would be OlOlE(1)E(1)E(2)E(2)m(1)m(1)m(2)m(2) for L-2890, OlOlE(1)E(1)E(2)E(2)M(1)M(1)M(2)M(2) for C-101, ololE(1)E(1)E(2)E(2)M(1)M(1)M(2)M(2) for L-1630, OlOle(1)e(1)e(2)e(2)M(1)M(1)M(2)M(2) for L-25X-1, ol1ollE(1)E(1)E(2)E(2)M(1)M(1)M(2)M(2) for L-482 and Ol1Ol1E(1)E(1)E(2)E(2)M(1)M(1)M(2)M(2) for L-1806. Transgressive recombinants were obtained from the cross L-1630 x L-25X-1, with about three-fold increase of the C 18: 1 content of the parents (> 643 g/kg) and free of C22: I content, which represent a high potential for commercial exploitation.