Large-scale phenotyping of ABR P1-N1 amplitudes before and after exposure to noise in 69 strains of mice.
Large-scale phenotyping of ABR P1-N1 amplitudes before and after exposure to noise in 69 strains of mice.
复制标题
对 69 种小鼠暴露于噪声前后的 ABR P1-N1 振幅进行大规模表型分析。
DOI：
10.1007/s00335-021-09913-0
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发表时间：
2021
期刊：
影响因子：
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通讯作者：
Friedman,RickAdam
中科院分区：
文献类型：
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作者：
Lavinsky,Joel;Mendonça,Aline;Bressan,Mariele;daSilva,VagnerAntonioRodrigues;Kasperbauer,Guilherme;Wang,Juemei;Salehi,Pezhman;Boussaty,ElyCheikh;Friedman,RickAdam
ABR波I振幅代表听神经纤维与内毛细胞的突触，与突触数量高度相关。耳蜗突触病，即内耳毛细胞和听神经纤维之间突触连接的丧失，已经在噪声性听力损失的动物模型中得到了很好的证实。在基线和噪声暴露后2周测定峰间波振幅。我们在8、12、16、24和32 kHz的频率下测定了80 dB SPL下的ABR波I幅值。共分析69株（1 ~ 8只/株）。在所有测试频率中，观察到波I振幅的统计显著后噪声降低（p< 0.00001）。我们确定了不同的噪声敏感性模式，并使这个完整的表型数据集可用于一般用途。这些数据为小鼠NIHL的研究提供了新的资源，我们希望该数据库能够成为扩展该领域研究的有用工具。
ABR wave I amplitude represents the synapse of auditory nerve fibers with the inner hair cell and is highly correlated with synapse counts. Cochlear synaptopathy, the loss of synaptic connections between inner hair cells and auditory nerve fibers, has been well-demonstrated in animal models of noise-induced hearing loss. The peak-to-peak wave I amplitude was determined at baseline and 2 weeks after noise exposure. We determined the ABR wave I amplitude at 80 dB SPL at the frequencies of 8, 12, 16, 24, and 32 kHz. A total of 69 strains (1–8 mice/strain) were analyzed. A statistically significant post-noise reduction in wave I amplitude was observed in all the tested frequencies (p< 0.00001). We identify distinct patterns of noise susceptibility and make this complete phenotypic dataset available for general use. This data establishes a new resource for the study of NIHL in mice and we hope this database will be a useful tool to expand the research in this field.