Deoxyglucose-6-phosphate stability in vivo and the deoxyglucose method.
Deoxyglucose-6-phosphate stability in vivo and the deoxyglucose method.
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6-磷酸脱氧葡萄糖体内稳定性和脱氧葡萄糖方法。
DOI：
10.1111/j.1471-4159.1987.tb02457.x
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发表时间：
1987
影响因子：
4.7
通讯作者：
Miller,AL
中科院分区：
文献类型：
--
作者：
Hawkins,RA;Miller,AL
P为DG。因此，在单次注射DG之后，在各种水解速率下，d（DGb）/dt= kT（DGp）-（k2+ kj）（DGb）+kj（DG-6-P）zyxwvutsrqponmlkj。如果DG-6-P从实验开始以仅1%/min的速率稳定地去除，则在45 min时净损失将为约24%;在3%/min时，损失将为约54%。即使是少量的G-6-P也会严重影响DG-6-P在所需的45分钟内被大脑保留。重要的问题是：（1）G-6-ATP是否存在于大脑中，如果存在，它在哪里，有多活跃？(2)G-6-β在体内是否与DG-6-P发生反应？(3)如果G-6-P是活性的，它在整个实验期间是否与DG-6-P反应？(4)G-6-β在整个大脑中分布均匀吗？(5)G-6-β活性在不同条件下是否不同？下文将讨论这些问题。
P to DG. Thus d (DGb)/dt= kT (DGp)-(k2+ kj)(DGb)+ k,(DG-6-P) zyxwvutsrqponmlkjih at various rates of hydrolysis after a single injection of DG. If DG-6-P were removed at the rate of only 1%/min steadily from the beginning of an experiment the net loss would be about 24% at 45 min; at 3%/min the loss would be about 54%. Even small amounts of G-6-Pase could seriously affect the retention of DG-6-P by brain during the required 45-min period. The important questions are:(1) Does G-6-Pase exist in brain and if so where and how active is it?(2) Does G-6-Pase react with DG-6-P in vivo?(3) If G-6-Pase is active, does it react with DG-6-P throughout the experimental period?(4) Is G-6-Pase distributed homogeneously throughout brain?(5) Does G-6-Pase activity vary under different conditions. These questions will be addressed below.