Chip-calorimetry provides real time insights into the inactivation of biofilms by predatory bacteria
Chip-calorimetry provides real time insights into the inactivation of biofilms by predatory bacteria
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DOI：
10.1080/08927014.2012.673593
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发表时间：
2012-03
期刊：
影响因子：
2.7
通讯作者：
F. Buchholz;J. Lerchner;F. Mariana;U. Kuhlicke;T. Neu;H. Harms;T. Maskow
中科院分区：
文献类型：
--
作者：
F. Buchholz;J. Lerchner;F. Mariana;U. Kuhlicke;T. Neu;H. Harms;T. Maskow
经常发现控制或去除不需要的生物膜是相当困难的。除了旨在防止生物污染的杀菌剂或抗生素化学品或材料之外，生物控制剂似乎也很有前景。关于细菌捕食者消除生物膜或消除病原体的报告促使人们对消除生物膜的细菌捕食者进行更系统的筛查。不幸的是，对根除过程的分析要求很高。在本研究中，应用芯片量热法监测假单胞菌的消除。 Bdellovibrio bacteriovorus 的生物膜。该方法使用代谢热作为生物膜活性的实时参数。该方法由于实时数据采集，非侵入性、快速、方便。此外，产热数据可以揭示捕食者与猎物相互作用的能量学信息。量热结果通过共焦激光扫描显微镜进行验证。所描述的方法可能有助于筛选生物膜对不同捕食者的敏感性。
Control or removal of undesired biofilms has frequently been found to be quite difficult. In addition to biocidal or antibiotic chemicals or materials designed to prevent biofouling, biological control agents appear to be promising. Reports of bacterial predators eradicating biofilms or eliminating pathogens motivate a more systematic screening of biofilm-eliminating bacterial predators. Unfortunately, the analysis of the eradication process is demanding. In the present study, chip-calorimetry was applied to monitor the elimination of Pseudomonas sp. biofilms by Bdellovibrio bacteriovorus. The method uses metabolic heat as a real-time parameter for biofilm activity. The method is non-invasive, fast and convenient due to real-time data acquisition. In addition, heat-production data can reveal information about the energetics of the predator–prey interaction. The calorimetric results were validated by confocal laser scanning microscopy. The approach described may be useful for the screening of biofilm susceptibility to different predators.