Adiponectin protects rat heart from left ventricular remodeling induced by chronic intermittent hypoxia via inhibition of TGF-β/smad2/3 pathway
Adiponectin protects rat heart from left ventricular remodeling induced by chronic intermittent hypoxia via inhibition of TGF-β/smad2/3 pathway
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DOI：
10.3978/j.issn.2072-1439.2014.07.44
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发表时间：
2014-09-01
影响因子：
2.5
通讯作者：
Lu, Gan
中科院分区：
文献类型：
--
作者：
Ding, Wen-Xiao;Dong, Yan-Bin;Lu, Gan
目的：阻塞性睡眠呼吸暂停综合征（OSAS）与多种心血管疾病相关。慢性间歇性缺氧（CIH）是OSAS中许多相关因素的主要参与者。本研究旨在探讨脂联素（Ad）对CIH所致左心室重构的影响。方法：将45只大鼠随机分为正常对照组（NC）、CIH组（CIH + Ad）和CIH + Ad补充组（CIH + Ad）。CIH暴露35 d后，采用masson分析检测左心室纤维化，western blot检测ⅰ型胶原、ⅲ型胶原及tgf - β /smad2/3通路蛋白表达。采用RT-PCR对MMP2和TIMP2进行基因分析。结果：CIH暴露后，CIH组左心室纤维化程度明显高于NC组和CIH + Ad组(P
Objective: Obstructive sleep apnea syndrome (OSAS) is associated with many cardiovascular disorders. Chronic intermittent hypoxia (CIH) is the primary player in OSAS of the many associated factors. This study was in order to investigate the effects of the Adiponectin (Ad) on left ventricular remodeling induced by CIH.Methods: Forty-five rats were randomly divided into three groups: normal control (NC) group, CIH group and CIH plus Ad supplemented (CIH + Ad) group. After 35 days' CIH exposure, masson analysis was used to detect the left ventricular fibrosis and western blot was used to measure the protein expression of collagen I, collagen III and TGF-beta/smad2/3 pathway. Gene analysis by RT-PCR was used to study the MMP2 and TIMP2.Results: After CIH exposure, the fibrosis of left ventricular in CIH group was significantly remarkable than that in both NC and CIH + Ad groups (P