Effect of storage and freezing on unbound bifirubin measurement
Effect of storage and freezing on unbound bifirubin measurement
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DOI：
10.1016/j.cca.2008.06.023
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发表时间：
2008-10-01
影响因子：
5
通讯作者：
Ahlfors, Charles
中科院分区：
文献类型：
--
作者：
Amin, Sanjiv B.;Ahlfors, Charles
背景：出生后未结合胆红素（B（f））比血清总胆红素（TSB）更能预测胆红素毒性。食品和药物管理局批准的用于B（f）分析的过氧化物酶试验目前在临床实验室中不可用。在进行B（f）analysis.Methods之前，通常将样本在不同温度和不同时间下储存，我们评估了将血液样本在-20 ℃和-80 ℃下储存长达1个月对B（f）测量的影响。在为入组前瞻性B（f）研究的新生儿采集的样本子集中，TSB（μ mol/l）和B（f）（μ mol/l），然后立即将样品在-20 ℃（n= 10）或-80 ℃（n= 10）下冷冻2周或在-20 ℃（n = 16）或-80 ℃（n = 10）下冷冻1个月。结果：-20 ℃和-80 ℃保存2周对B（f）测定无影响。在-80 ℃下储存一个月后，似乎对B（f）没有影响。结论：本研究结果表明，用于B（f）测定的血液样本应在采集并在-20 ℃下储存后2周内进行分析。(c)2008 Elsevier B. V.保留所有权利。
Background: Unbound bilirubin (B(f)) is a better predictor of bilirubin toxicity than total serum bilirubin (TSB) in the postnatal period. The Food and Drug Administration approved peroxidase test for B(f) analysis is not currently available in clinical laboratories. The samples are often stored at varying temperature and for varying duration before B(f) analysis.Methods: We evaluated the effect of storing blood samples up to 1 month at -20 degrees C and -80 degrees C on B(f) measurement. In a subset of samples collected for neonates enrolled in a prospective B(f) study, TSB (mu mol/l) and B(f) (mu mol/l) were measured by the peroxidase test and then the samples were immediately frozen at -20 degrees C (n= 10) or -80 degrees C (n= 10) for either 2 weeks or at -20 degrees C (n = 16) or -80 degrees C (n = 10) for 1 month. After the designated freezing period, the samples were thawed and TSB and B(f) measured again.Results: There was no effect of storage for 2 weeks at either -20 degrees C or -80 degrees C on B(f) measurement. There appears to be no effect on B(f) after storage at -80 degrees C for a month. However, there was significant decrease in B(f) after storage at -20 degrees C for a month.Conclusions: The results of this study suggest that blood samples for B(f) measurement should be analyzed within 2 weeks after collection and storage at -20 degrees C. (c) 2008 Elsevier B.V. All rights reserved.