High-Throughput MicroRNA and mRNA Sequencing Reveals That MicroRNAs May Be Involved in Melatonin-Mediated Cold Tolerance in Citrullus lanatus L.
High-Throughput MicroRNA and mRNA Sequencing Reveals That MicroRNAs May Be Involved in Melatonin-Mediated Cold Tolerance in Citrullus lanatus L.
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DOI：
10.3389/fpls.2016.01231
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发表时间：
2016
影响因子：
5.6
通讯作者：
Zhang X
中科院分区：
文献类型：
--
作者：
Li H;Dong Y;Chang J;He J;Chen H;Liu Q;Wei C;Ma J;Zhang Y;Yang J;Zhang X
冷应答基因的转录调控在外源褪黑激素介导的植物耐冷性中起着重要作用。尽管如此，褪黑激素如何调节冷反应基因在很大程度上是未知的。在本研究中，我们发现外源褪黑激素通过调节microRNA（miRNAs）的表达来提高西瓜的耐冷性。我们鉴定了一组在非应激或寒冷条件下受褪黑激素调控的miRNAs。重要的是，mRNA-seq分析显示，褪黑激素诱导的一些miRNAs，如miR 159 - 5 p，miR 858，miR 8029 - 3 p和novel-m0048- 3 p的下调与参与冷胁迫下信号转导（CDPK，BHLH，WRKY，MYB和DREB）和保护/解毒（莱亚和MDAR）的靶基因的上调相关。这些结果表明，miRNAs可能通过负调控靶mRNA的表达而参与了褪黑激素介导的西瓜耐冷性。
Transcriptional regulation of cold-responsive genes is crucial for exogenous melatonin-mediated cold tolerance in plants. Nonetheless, how melatonin regulates cold-responsive genes is largely unknown. In this study, we found that exogenous melatonin improved cold tolerance in watermelon by regulating expression of microRNAs (miRNAs). We identified a set of miRNAs that were regulated by melatonin under unstressed or cold conditions. Importantly, mRNA-seq analysis revealed that melatonin-induced downregulation of some miRNAs, such as miR159-5p, miR858, miR8029-3p, and novel-m0048-3p correlated with the upregulation of target genes involved in signal transduction (CDPK, BHLH, WRKY, MYB, and DREB) and protection/detoxification (LEA and MDAR) under cold stress. These results suggest that miRNAs may be involved in melatonin-mediated cold tolerance in watermelon by negatively regulating the expression of target mRNAs.