Purification, structural characterization, and immunomodulatory activity of the polysaccharides from Ganoderma lucidum
Purification, structural characterization, and immunomodulatory activity of the polysaccharides from Ganoderma lucidum
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DOI：
10.1016/j.ijbiomac.2019.09.141
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发表时间：
2020-01-15
影响因子：
8.2
通讯作者：
Yu, Guangli
中科院分区：
文献类型：
--
作者：
Li, Jia;Gu, Feifei;Yu, Guangli
初步阐明了灵芝多糖的结构特征与相应的免疫调节活性之间的关系。采用乙醇梯度沉淀法和Q-SepharoseFast Flow(QFF)强阴离子交换柱从灵芝提取液中分离纯化了两种多糖(GLP-1和GLP-2)。用单糖组成、高效凝胶渗透色谱-多角度激光散射-折射率(HPGPC-MALLS-RI)、傅立叶变换红外光谱(FT-IR)、原子力显微镜(AFM)、甲基化分析和核磁共振(NMR)对其进行了表征。GLP-1多糖被鉴定为半乳糖基葡聚糖，具有灵活的随机线性构象，主要由--D-Glcp-(1->；，->；6)-α-D-Galp-(1->；)和->；3-β-D-Glcp-(1->；残基)组成。GLP-2是一种相对均一的β-D-葡聚糖，具有-β-D-Glcp-(1-gt；和->；3)-β-D-Glcp-(1->；6)-β-D-Glcp-(1->；)残基。体内免疫调节活性表明，GLP-1对脾和胸腺有较好的保护作用，对促进造血和提高血清中IgA水平有较好的作用。我们的结果表明，灵芝多糖的免疫调节活性与其碳水化合物组成、分子量和高级构象等结构特征高度一致。本研究为研究多糖结构特征与药理活性的关系提供了初步依据。(C)2019爱思唯尔B.V.保留所有权利。
A preliminary relationship was illustrated between the structural characteristics and corresponding immunomodulatory activities of G. lucidum polysaccharides. Two polysaccharides (GLP-1 and GLP-2) were purified from Ganoderma lucidum extracts by gradient ethanol precipitation and a Q-Sepharose Fast Flow (QFF) strong anion-exchange column. The monosaccharide composition, high-performance gel permeation chromatography-multi-angle laser light scattering-refractive index (HPGPC-MALLS-RI), Fourier-transform infrared spectroscopy (FT-IR), atomic force microscopy (AFM), methylation analysis, and nuclear magnetic resonance (NMR) were used to characterize these polysaccharides. The GLP-1 polysaccharide was elucidated as Dgalactoglucan with a flexible random linear conformation that mainly composed of -> 6)beta-D-Glcp-(1 ->,-> 6)alpha-D-Galp-(1 ->, and -> 3)-beta-D-Glcp-(1 -> residues. GLP-2 was found to be a relatively homogeneous beta-D-glucan that possessing->6)-beta-D-Glcp-(1 -> and -> 3)-beta-D-Glcp-(1 -> residues packaged into a spherical conformation. immunomodulatory activities in vivo demonstrated that GLP-1 produced better protection of the spleen and thymus and was more effective for promoting hematopoiesis and improving IgA levels in serum. Our results suggest that the immunomodulatory activities of G. lucidum polysaccharides are highly corresponded to their structural characteristics such as carbohydrate composition, molecular weight and advanced conformation. This study provides a preliminary basis for studying the relationship between polysaccharide structure characterization and pharmacological activities. (C) 2019 Elsevier B.V. All rights reserved.