The modulation of apoptosis by cyclic AMP involves Akt and epidermal growth factor receptor.
The modulation of apoptosis by cyclic AMP involves Akt and epidermal growth factor receptor.
复制标题
环AMP对细胞凋亡的调节涉及Akt和表皮生长因子受体。
DOI：
10.1016/j.biocel.2005.02.012
复制
发表时间：
2005
期刊：
影响因子：
--
通讯作者：
Jianguo Song
中科院分区：
文献类型：
--
作者：
Bo Zhou;Fuqiang Li;Hehua Chen;Jianguo Song
环磷酸腺苷（cAMP）和转化生长因子-β（TGF-β）是许多生物过程的重要调节因子。本研究探讨了cAMP对转化生长因子-β1和血清剥夺诱导的Mv 1 Lu细胞凋亡的影响及其潜在机制。转化生长因子-β1处理或血清剥夺诱导Mv 1 Lu细胞凋亡反应。cAMP升高剂Forskolin或cAMP类似物8-Bromo-cAMP（8-B-cAMP）可明显抑制转化生长因子β1诱导的细胞增殖和凋亡，但完全抑制血清剥夺诱导的细胞凋亡。Forskolin可降低Akt的磷酸化水平，LY 294002对磷脂酰肌醇-3激酶的抑制可使Mv 1 Lu细胞对转化生长因子-β1诱导的凋亡敏感。此外，毛喉素治疗诱导表皮生长因子受体的酪氨酸磷酸化。特异性抑制剂PD 153035抑制表皮生长因子受体阻断cAMP介导的抑制血清剥夺诱导的细胞凋亡。结果表明，cAMP通过调节Mv 1 Lu细胞中磷脂酰肌醇-3-激酶/Akt和表皮生长因子受体的信号成分，在转化生长因子-β1和血清剥夺诱导的细胞凋亡中发挥相反的作用。
Adenosine 3′,5′-cyclic monophosphate (cAMP) and transforming growth factor-β are important regulators of many biological processes. In this study we investigated the effect and its potential mechanism of cAMP on transforming growth factor-β1- and serum deprivation-induced apoptosis in Mv1Lu cells. Transforming growth factor-β1 treatment or serum deprivation induces apoptotic response in Mv1Lu cells. Forskolin, a cAMP-elevating agent, or 8-Bromo-cAMP (8-B-cAMP), a cell permeable cAMP analogue, inhibited the cell proliferation and markedly enhanced apoptosis induced by transforming growth factor-β1, but completely suppressed serum deprivation-induced apoptosis. Furthermore, forskolin decreased the Akt phosphorylation, and the inhibition of phosphatidylinositol-3 kinase by LY294002 sensitized Mv1Lu cells to transforming growth factor-β1-induced apoptosis. In addition, forskolin treatment induced tyrosine phosphorylation of epidermal growth factor receptor. Inhibition of epidermal growth factor receptor by specific inhibitor PD153035 blocked the cAMP-mediated suppression of serum deprivation-induced apoptosis. The results indicate that cAMP exerts its opposite effects in transforming growth factor-β1- and serum deprivation-induced apoptosis via a mechanism involving the modulation of signaling components of phosphatidylinositol-3-kinase/Akt and epidermal growth factor receptor in Mv1Lu cells.
登录
查看更多内容
垂体腺苷酸环化酶激活多肽抑制人垂体腺瘤细胞系中转化生长因子-β1 诱导的细胞凋亡。
DOI：
10.1016/s0002-9440(10)65509-5
发表时间：
1999
期刊：
The American journal of pathology
影响因子：
--
作者：
Oka,H;Jin,L;Kulig,E;Scheithauer,BW;Lloyd,RV
通讯作者：
Lloyd,RV
胚胎腭细胞中 cAMP、TGF-β 和视黄酸信号通路的汇聚。
影响因子：
6.1
作者：
Nugent,P;Kusek,JC;Pisano,MM;Greene,RM
通讯作者：
Greene,RM
通过转化生长因子-β1 和表皮生长因子协同诱导环氧合酶-2，抑制上皮细胞凋亡。
DOI：
10.1038/sj.neo.7900051
发表时间：
1999
期刊：
Neoplasia (New York, N.Y.)
影响因子：
--
作者：
Saha,D;Datta,PK;Sheng,H;Morrow,JD;Wada,M;Moses,HL;Beauchamp,RD
通讯作者：
Beauchamp,RD
影响因子：
3.3
作者：
Jost, M;Huggett, TM;Rodeck, U
通讯作者：
Rodeck, U
影响因子：
3.3
作者：
Giri, D;Ittmann, M
通讯作者：
Ittmann, M