Two retroviruses packaged in one cell line can combined inhibit the replication of HIV-1 in TZM-bl cells
Two retroviruses packaged in one cell line can combined inhibit the replication of HIV-1 in TZM-bl cells
复制标题
DOI：
10.1007/s12250-012-3263-8
复制
发表时间：
2012-11
影响因子：
5.5
通讯作者：
Zhipin Liang;Zhi Guo;Xin Wang;Xiaohong Kong;Chang Liu
中科院分区：
文献类型：
--
作者：
Zhipin Liang;Zhi Guo;Xin Wang;Xiaohong Kong;Chang Liu
细胞蛋白质系链蛋白将HIV-1病毒颗粒系在细胞膜上以抑制HIV-1的复制。然而，HIV-1辅助蛋白Vpu抵消了tetherin的抗病毒功能。本研究构建了两个逆转录病毒载体质粒。一个抑制Vpu基因表达，另一个过度表达tetherin。两种逆转录病毒载体质粒均可在包装细胞系PT 67中包装，获得相应的逆转录病毒。在细胞水平上检测逆转录病毒载体质粒的tetherin过表达或vpu-RNAi功能。将逆转录病毒载体质粒以0 T3 V ~ 3 T0 V的不同比例转染PT 67细胞，然后收获混合逆转录病毒。在HIV-1感染的TZM-bl细胞中检测了混合逆转录病毒的抗病毒作用。结果表明，包装的混合逆转录病毒能抑制HIV-1在TZM-bl细胞中的复制。
The cellular protein tetherin tethers the HIV-1 viral particles on the cellular membrane to inhibit the replication of HIV-1. However, the HIV-1 accessory protein Vpu counteracts the antiviral function of tetherin. In this study, two retroviral vector plasmids were constructed. One inhibited thevpugene expression; the other one over-expressed the tetherin. Both retroviral vector plasmids could be packaged in the packaging cell line PT67 to obtain the corresponding retroviruses. The retroviral vector plasmids’ functions of tetherin over-expression or vpu-RNAi were detected at the cell level. Retroviral vector plasmids were transfected to PT67 cells at different ratios from 0T3V to 3T0V, and then mixed retroviruses were harvested. The antiviral functions of mixed retroviruses were detected in HIV-1 infected TZM-bl cells. The results showed that packaged mixed retroviruses could repress the replication of HIV-1 in TZM-bl cells.