Long-term measurement of cerebral blood flow and metabolism in a rat chronic hypoperfusion model
Long-term measurement of cerebral blood flow and metabolism in a rat chronic hypoperfusion model
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DOI：
10.1046/j.1440-1681.2003.03825.x
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发表时间：
2003-04-01
影响因子：
2.9
通讯作者：
Terashi, A
中科院分区：
文献类型：
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作者：
Otori, T;Katsumata, T;Terashi, A
1.采用大鼠双侧颈总动脉结扎(BCAO)作为慢性脑低灌注模型。用放射自显影技术观察BCAO后2天、1、4、8周及对照组局部脑血流量(RCBF)和局部脑葡萄糖利用率(RCGU)的长期变化。被评估的区域包括大脑皮层、白质和基底节。采用Kluver-Barrera和苏木精-伊红染色观察病理变化。2天后，皮质、白质和杏仁核的rCBF明显下降到33-58%，1周后也有类似的下降。4周后，rCBF恢复，但枕叶皮质、白质、苍白球和黑质的rCBF仍明显减少。2d后rCGU基本维持，1周后皮质、白质、基底节和丘脑的rCGU明显减少至40%~70%。四周后，这些削减再也看不到了。1周后脑白质稀疏。结果表明，大鼠局灶性脑缺血是一种较好的慢性脑低灌注模型，脑白质内rCBF和rCGU在2d至4周出现解偶联现象。
1. Rat bilateral common carotid artery occlusion (BCAO) was used as a chronic cerebral hypoperfusion model. We observed autoradiographically the long-term changes in regional cerebral blood flow (rCBF) and regional cerebral glucose utilization (rCGU) after 2 days and 1, 4 and 8 weeks of BCAO and in controls. Regions evaluated included the cerebral cortex, white matter and basal ganglia. Pathological changes were also observed with Kluver-Barrera and haematoxylin-eosin staining.2. After 2 days, rCBF was significantly reduced to 33-58% in the cortex, white matter and amygdala and similar reductions were observed after 1 week.3. After 4 weeks, rCBF recovered; however, rCBF remained significantly reduced in the occipital cortex, white matter, globus pallidus and substantia nigra.4. After 2 days, rCGU was mostly maintained but, after 1 week, rCGU was reduced significantly to 40-70% in the cortex, white matter, basal ganglia and thalamus. Four weeks later, these reductions were no longer seen.5. Rarefaction of the white matter was observed from 1 week.6. These results showed that the BCAO in rats is an appropriate model for chronic cerebral hypoperfusion and that uncoupling of rCBF and rCGU was observed from 2 days until 4 weeks in the white matter.