Direct sampling and analysis from solid-phase extraction cards using an automated liquid extraction surface analysis nanoelectrospray mass spectrometry system
Direct sampling and analysis from solid-phase extraction cards using an automated liquid extraction surface analysis nanoelectrospray mass spectrometry system
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DOI：
10.1002/rcm.5132
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发表时间：
2011-09-15
影响因子：
2
通讯作者：
Van Berkel, Gary J.
中科院分区：
文献类型：
--
作者：
Walworth, Matthew J.;ElNaggar, Mariam S.;Van Berkel, Gary J.
基于直接液体萃取的表面采样，一种先前用连续流动和自主移液管液体微结表面采样探针证明的技术，最近已在市售的基于芯片的输注纳米电喷雾电离（nanoESI）系统上作为液体萃取表面分析（LESA）模式实施。在本文中，LESA模式应用于分析96孔格式定制的固相萃取（SPE）卡，每个孔由一个1或2毫米直径的整体式疏水固定相组成。调节这些底物威尔斯孔，加载单组分或多组分水性混合物，并使用与混合三重四极杆/线性离子阱质谱仪或线性离子阱质谱仪偶联的商业nanoESI系统读出。在目标化合物的分析中优化了提取条件，包括提取/nanoESI溶剂组成、体积和停留时间。测定了检测限和定量限以及分析重现性优值。使用氘代内标物获得普萘洛尔的校准数据，证明其线性和重现性。证明了信号增加10倍，并从浓盐溶液中清除了微摩尔血管紧张素II。此外，在存在同量异位素干扰的情况下，使用MS（3）光谱分析ppb浓度水平的多组分除草剂混合物，用于化合物鉴定。出版社：John Wiley & Sons，Ltd
Direct liquid extraction based surface sampling, a technique previously demonstrated with continuous flow and autonomous pipette liquid microjunction surface sampling probes, has recently been implemented as a liquid extraction surface analysis (LESA) mode on a commercially available chip-based infusion nanoelectrospray ionization (nanoESI) system. In the present paper, the LESA mode was applied to the analysis of 96-well format custom-made solid-phase extraction (SPE) cards, with each well consisting of either a 1 or a 2mm diameter monolithic hydrophobic stationary phase. These substrate wells were conditioned, loaded with either single or multi-component aqueous mixtures, and read out using the commercial nanoESI system coupled to a hybrid triple quadrupole/linear ion trap mass spectrometer or a linear ion trap mass spectrometer. The extraction conditions, including extraction/nanoESI solvent composition, volume, and dwell times, were optimized in the analysis of targeted compounds. Limit of detection and quantitation as well as analysis reproducibility figures of merit were measured. Calibration data was obtained for propranolol using a deuterated internal standard which demonstrated linearity and reproducibility. A 10x increase in signal and cleanup of micromolar angiotensin II from a concentrated salt solution was demonstrated. In addition, a multicomponent herbicide mixture at ppb concentration levels was analyzed using MS(3) spectra for compound identification in the presence of isobaric interferences. Published in 2011 by John Wiley & Sons, Ltd.