High Variation in Single Nucleotide Polymorphisms (SNPs) and Insertions/Deletions (Indels) in the Highly Invasive Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) Middle East-Asia Minor 1 (MEAM1)
High Variation in Single Nucleotide Polymorphisms (SNPs) and Insertions/Deletions (Indels) in the Highly Invasive Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) Middle East-Asia Minor 1 (MEAM1)
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高入侵性烟粉虱 (Gennadius)（半翅目：粉虱科）中东小亚细亚 1 (MEAM1) 中单核苷酸多态性 (SNP) 和插入/缺失 (Indels) 的高度变异
DOI：
10.1007/s13744-013-0152-2
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发表时间：
2013-10-01
影响因子：
1.8
通讯作者：
Zhang, G. F.
中科院分区：
文献类型：
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作者：
Lu, Z. C.;Sun, H. B.;Zhang, G. F.
烟粉虱（Bemisiatabaci（Gennadius））（半翅目：粉虱科）中东-小亚细亚1号（MEAM 1）是一种入侵性强、适应性广的害虫。适应性与单核苷酸多态性（SNP）和插入/缺失（indels）等基因组变异密切相关。为了阐明MEAM 1中SNPs和indel的特征，并揭示SNPs/indel与不同环境适应能力之间的关系，基于NCBI数据库中的9110个MEAM 1表达序列标签，采用QualitySNP计算方法鉴定可靠的SNPs和indel。共检测到575个SNPs，SNPs密度为10.1个SNPs/kb和6.4个SNPs/contig。此外，获得了237个转换（39.3%）和366个颠换（60.7%），其中转换与颠换的比率为0.65：1。另外，检测到581个indel，密度为14.1 indel/kb和9.2 indel/contig。总的来说，这表明侵袭性MEAM 1具有高SNPs密度，并且SNPs百分比高于非侵袭性B。烟粉虱种类MEAM 1中的高SNPs密度/百分比产生了高基因组变异，这可能使其能够适应不同的环境，这为在基因组水平上理解MEAM 1的侵袭性提供了一些支持。高水平的基因组变异与适应能力水平有关，入侵物种被认为比非入侵物种表现出更高水平的适应能力。
Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) Middle East-Asia Minor 1 (MEAM1) is invasive and adaptive to varied environments throughout the world. The adaptability is closely related to genomic variation such as single nucleotide polymorphisms (SNPs) and insertions/deletions (indels). In order to elucidate the feature of SNPs and indels in MEAM1, and reveal the association between SNPs/indels and adaptive capacity to various environments, a computational approach with QualitySNP was used to identify reliable SNPs and indels on the basis of 9110-expressed sequence tags of MEAM1 present in the NCBI database. There were 575 SNPs detected with a density of 10.1 SNPs/kb and 6.4 SNPs/contig. Also, 237 transitions (39.3%) and 366 transversions (60.7%) were obtained, where the ratio of transitions to transversions was 0.65:1. In addition, 581 indels with a density of 14.1 indels/kb and 9.2 indels/contig were detected. Collectively, it showed that invasive MEAM1 has high SNPs density, and higher SNPs percentage than non-invasive B. tabaci species. A high SNPs density/percentage in MEAM1 yielded a high genomic variation that might have allowed it to adapt to varied environments, which provides some support to understand the invasive nature of MEAM1 at the genomic level. High levels of genomic variation are implicated in the level of adaptive capacity and invasive species are thought to exhibit higher levels of adaptive capacity than non-invasive species.