Endogenous 15-deoxy-Δ12,14-prostaglandin J2 synthesized by adipocytes during maturation phase contributes to upregulation of fat storage
Endogenous 15-deoxy-Δ12,14-prostaglandin J2 synthesized by adipocytes during maturation phase contributes to upregulation of fat storage
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DOI：
10.1016/j.febslet.2006.11.049
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发表时间：
2006-12-22
期刊：
影响因子：
3.5
通讯作者：
Yokota, Kazushige
中科院分区：
文献类型：
--
作者：
Mazid, Md. Abdul;Asad Chowdhury, Abu;Yokota, Kazushige
15-脱氧-Delta(12,14)-前列腺素 J(2) (15d-PGJ(2)) 已被确定为过氧化物酶体增殖物激活受体 (PPAR) γ 的天然配体，可促进脂肪形成。然而，表达PPARγ的脂肪细胞产生PGJ(2)系列的能力以及在脂肪细胞生命周期中的作用仍然难以捉摸。在这里，我们开发了一种针对 15d-PGJ(2) 的酶联免疫吸附测定法。使用该方法的分析揭示了在成熟阶段培养的脂肪细胞中免疫反应性15d-PGJ(2) 的内源合成增加。使用环氧合酶抑制剂的进一步研究阐明了成熟脂肪细胞产生的内源性 15d-PGJ(2) 对以自分泌方式上调脂肪储存的贡献。 (c) 2006 年欧洲生化学会联合会。由 Elsevier B.V. 出版。保留所有权利。
15-Deoxy-Delta(12,14)-prostaglandin J(2) (15d-PGJ(2)) has been identified as a natural ligand for peroxisome proliferator-activated receptor (PPAR) gamma to promote adipogenesis. However, it remains elusive about the ability of PPAR gamma-expressing adipocytes to produce PGJ(2) series and the role in the life cycle of adipocytes. Here, we developed an enzyme-linked immunosorbent assay specific for 15d-PGJ(2). The analysis using this method revealed the increase in the endogenous synthesis of immunoreactive 15d-PGJ(2) in cultured adipocytes during the maturation phase. Further studies using cyclooxygenase inhibitors clarified the contribution of endogeous 15d-PGJ(2) produced by mature adipocytes to upregulation of fat storage in an autocrine manner. (c) 2006 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.